400P - LncRNA-LINC00294 functions as a ceRNA in regulating JUP through competitively binding to miR-485-5p in breast cancer

Background

Long non-coding RNAs (LncRNAs) play a critical part in tumor growth and metastasis, but only a small number of LncRNAs are functionally annotated.

Methods

The GEO database was used to analyze breast cancer-related transcriptome data and identify the differentially expressed LncRNA-LINC00294, and qRT-PCR was used to validate the expression of LINC00294 in 30 pairs of breast and para cancer tissues, as well as normal human breast epithelial cells MCF-10A and breast cancer cells MDA-MB-231, SK-BR-3, and MCF-7. The influence of LINC00294 overexpression/interference on the biological properties of breast cancer cells was validated using Western Blot, Transwell, scratch assay, flow cytometry, and CCK-8; the infection of LINC00294 overexpression on the growth of breast tumors in vivo was validated using an in situ tumorigenesis assay in nude mice; the effects of LINC00294 overexpression on the growth of breast tumors in vivo were inquired using Western Blot. The infection of LINC00294 in breast cancer cell biology was investigated by Western Blot, Transwell, scratch assay, and luciferase reporter to investigate the mechanism by which LINC00294 affects breast cancer development via ceRNA adsorption and thus regulates JUP expression.

Results

LINC00294 was significantly upregulated in breast cancer tissues. Furthermore, interference with LINC00294 significantly inhibited breast cancer cell multiplication, invasion, and migration, while overexpression of LINC00294 promoted breast cancer cell invasion proliferation, proliferation, and migration, as well as tumor growth. According to mechanistic studies, LINC00294 could regulate JUP expression and thus the Wnt/β-catenin signaling pathway by targeting miR-485-5p, thus affecting the development of breast cancer.

Conclusions

LINC00294 is highly expressed in breast cancer and may spaciously adsorb miR-485-5p through the ceRNA mechanism to regulate JUP expression, which in turn regulates the Wnt/β-catenin signaling pathway and affects the development of breast cancer. Our findings show that LINC00294 has an effect on breast cancer and identifies a new possible prognostic marker and therapeutic target for this disease.

Clinical trial identification

Editorial acknowledgement

Legal entity responsible for the study

The authors.

Funding

the 'Kuanren meritocrat'Backbone Talents Project of The Second Affiliated Hospital of Chongqing Medical University (no. KY2019G016), Wu Jiping Medical Foundation (no.320.6750.2021-10-86) and Natural Science Foundation of Chongqing, China(no.CSTB2022NSCQ-MSX0055).

Disclosure

All authors have declared no conflicts of interest.