RT-PCR detects NTRK gene fusions expressed at the RNA level . RT-PCR detects a single fusion if the involved exons of both genes are known. However, multiple transcripts can be covered with a type of RT-PCR known as nested RT-PCR.
- Argani P, Fritsch M, Kadkol SS et al. Detection of the ETV6-NTRK3 chimeric RNA of infantile fibrosarcoma/cellular congenital mesoblastic nephroma in paraffin-embedded tissue: application to challenging pediatric renal stromal tumors. Mod Pathol 2000; 13: 29-36.