Abstract 101P
Background
After invasive ductal carcinoma, invasive lobular carcinoma (ILC) is the most frequent breast cancer and accounts for nearly 15% of all breast cancers (BC). Doxorubicin (DXR) is a very effective chemotherapeutic drug utilized to treat BC but induces drug resistance and severe side effects. Recently, liposomes have gained popularity as effective tumor microenvironment (TME) modulatory anticancer agents for various malignancies, including BC. This study propose a liposomal-DXR combination to produce an immune-responsive regulatory effect on competing endogenous RNAs (ceRNAs) through alteration in TME and investigate the immunological and anticancer impact of liposomal-DXR combination via regulation of ceRNAs and immune regulatory cytokines at BC cells.
Methods
MDA-MB cells were treated with varying concentration of liposomal-DXR combination and dose response curve was calculated. Total RNA was extracted and quantified by qRT-PCR and TNF-α ELISA kit. Computational target interaction was analyzed.
Results
Treatment resulted in a dose and time-dependent cell-viability and migration inhibition. Treatment altered the level of AKT1, ESR1, and ARID1A genes. Alteration in miR-17-5p compared to control cells indicated the alteration in ceRNAs network. TNF-α was found repressed in treated MDA-MB cells.
Conclusions
Present study propose the mechanism of liposomal-DXR as potential TME modulatory, immunoregulatory anti-ILC agent through altering ceRNAs circuit, AKT1, ESR1, and ARID1A genes, and immune regulatory TNF-α in BC cells.
Legal entity responsible for the study
The author.
Funding
Has not received any funding.
Disclosure
The author has declared no conflicts of interest.