Abstract 180P
Background
Schwann cells(SC), as the significant component of the peripheral nervous system, have been shown to affect tumor microenvironment in many kinds of cancer.The interaction between SC and cancer has been demonstrated in many kinds of cancer, such as prostate cancer, pancreatic cancer and non-small cell lung cancer. But in small cell lung cancer(SCLC), it is still unclear.
Methods
To clarify this issue, we conducted cell proliferation assay, Annexin V apoptosis assay and Transwell migration and invasion assay, and elucidated SCs have a well-established role in facilitating cancer migration and invasion of SCLC. We then investigated the proposed mechanism by screen the different expression genes(DEGs) and constructing mRNA-miRNA-lncRNA networks of DMS114 stimulated by DMS114- activated SCs, SCs and blank medium. Then, we performed a survival analysis of DEGs based on TCGA LUAD and LUSC profile.
Results
In general, significant DEG were observed between any two groups.First, we made comparison of DEG expression in the SC/DMS114-conditioned group and control group. There were 10 significant DEGs, including up-regulated DEG: STAT3, SOCS3, BCL6, ELF3, IGF2, IL32, RUNX1, and NEUROD2, and down-regulated DEG: CHAC2 and BOLA2B.the significant DEGs between the SC-conditioned group and control group were also including STAT3, SOCS3, BCL6, ELF3, IGF2, IL32. Other than these DEGs, up-regulated of KRT6A, PLA2G2A, CEACAM1, KRT14, SPEM2,GABRP, and A2M, as well as down-regulated of ACTA1 and MYH7 also involved in DEGs of two groups. The significant DEGs in SC/DMS114-conditioned group and SC-conditioned group were both enriched in Notch signaling pathway(GO:0007219) and regulation of Notch signaling pathway(GO:0008593).Finally,we found expression of GABRP was significantly higher in SC activated SCLC, and high expression of GABRP associated with worse survival outcome(p=0.0083).
Conclusions
SC may promote tumor progression in SCLC. In SC activated SCLC, expression of GABRP was unregulated and high expression of GABRP was related with poor outcome in lung cancer.
Legal entity responsible for the study
Hua Zhong.
Funding
Has not received any funding.
Disclosure
All authors have declared no conflicts of interest.