Abstract 120P
Background
Melanoma is aggressive cancer metastasizing to various organs, including the liver and lungs. One of the characteristics of melanoma is the ability to synthesize melanin. In in vitro cultures, the melanin synthesis process by melanoma cells seems to be disrupted. Our aim is to present how melanoma cell pigmentation influences tumor development.
Methods
In our research, we used human uveal melanoma cell lines isolated from patients. Melanoma cells were grown in a medium stimulating (addition of L-tyrosine) and not stimulate pigmentation. Under both conditions, a cell proliferation assay was performed. Cell migration was determined using the wound healing assay. The ability of melanoma cells to form capillary-like structures was measured. The CAM (chick chorioallantoic membrane) model was used as the in vivo model. Melanoma cells were implanted on the chorioallantoic membrane. Tumor growth was observed over the next 6 days. The development of the obtained tumors and the level of pigmentation were determined in the CAM model. Histological analysis of the collected tumors was performed.
Results
In the in vitro model, we observe that the stimulation of pigmentation reduces the ability of cells to form capillary-like structures. A change in cell migration is also seen. We do not observe a change in proliferation. In the CAM model, cell lines do not lose their pigmentation ability.
Conclusions
Pigmentation of melanoma cells causes changes in cell migration and the ability to form capillary-like structures. The CAM model used reflects the ability of melanoma cells to pigmentation, which allows the continuation of in vivo research.
Clinical trial identification
Editorial acknowledgement
Legal entity responsible for the study
The authors.
Funding
Has not received any funding.
Disclosure
All authors have declared no conflicts of interest.