Abstract 16P
Background
IL-8 gene polymorphisms are involved in the carcinogenesis of many malignant neoplasms, but their role in the development of cervical cancer (CC) has not been fully elucidated. The aim of the study was to evaluate the relationship between the presence of IL-8 gene polymorphism (845 T/C) and the development of severe cervical intraepithelial neoplasia (CIN) and cervical cancer.
Methods
The study included 21 patients with CIN III and stage Ia FIGO cervical cancer and 20 healthy women (control). The study was conducted in accordance with the requirements of the USU Ethics Commission (No. 9 dated September 15, 2016). We determined the level of IL-8 (CJSC Vector-Best-Volga, Russia) using ELISA in neutrophil lysate and serum. For the analysis of SNP IL-8 845 T/C (rs2227532), the samples were genotyped by PCR with restriction enzyme analysis using Vsp I endonuclease (SibEnzyme, Russia). The digested PCR products were separated by electrophoresis in 1.5% agarose gel. Statistical processing was carried out using Statistica 13 (StatSoft).
Results
We found that the -845C* allele was more common in the group with cervical lesions (76.2%) than in the control group (10%) (chi-square=18.223, p=0.001). The risk of developing CIN increased in the presence of the -845C* allele (OR=28.8, 95% CI 4.892-169.546, p=0.038). The level of IL-8 in neutrophils in CIN (p=0.015) was lower than that in the control. At the same time, the level of IL-8 in neutrophils and serum in CIN is not associated with the frequency of occurrence of the -845T* and -845C alleles.
Conclusions
The presence of IL-8 gene polymorphism (845 T/C) is associated with CIN.
Legal entity responsible for the study
The authors.
Funding
Has not received any funding.
Disclosure
All authors have declared no conflicts of interest.