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ePoster Display

34P - The interrelations between CLIC 1 expression and the histological growth pattern of liver metastases of digestive origin


16 Sep 2021


ePoster Display


Basic Science

Tumour Site


Amalia Ceausu


Annals of Oncology (2021) 32 (suppl_5): S361-S375. 10.1016/annonc/annonc684


A.R. Ceausu1, A.A. Cosma1, A. Ciolofan2, P.N. Gaje1, S.M. Negru3, O.M. Cretu2, M. Raica1

Author affiliations

  • 1 Microscopic Morphology/histology/angiogenesis Research Center, "Victor Babes" University of Medicine and Pharmacy, 300041 - Timisoara/RO
  • 2 Department Of Surgery, "Victor Babes" University of Medicine and Pharmacy, 300041 - Timisoara/RO
  • 3 Department Of Oncology, OncoHelp Oncology Center Timisoara, 300239 - Timisoara/RO


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Abstract 34P


Chloride intracellular channel 1 (CLIC1) is expressed by several malignancies, and it is involved in cell migration and metastasis. The histological growth pattern (HGP) such as desmoplastic (DGP), replacement (RGP), pushing (PGP) and mixed has been noticed in the liver metastases of colorectal, pancreatic, gastric, breast and uveal melanoma origin. The aim of this abstract is to determine the CLIC 1 immunoexpression variability amongst different liver metastasis HGP.


We included in the study primary tumors from colorectal (15), pancreatic (7) and gastric cancer (2 cases), and their corresponding liver metastasis (LM). The full immunohistochemical procedure was performed with Bond Max autostainer. The CLIC 1 reaction was evaluated according with the following score: 0 (less than 10% positive cells), 1(10-30%), 2 (30-50%) and score 3 (more than 50% CLIC 1 positive cells).


All LM expressed CLIC 1 heterogeneously. CLIC 1 intensity score values varied between 1 and 3. CLIC 1 positive liver sinusoidal endothelial cells and cells with starry-like morphology were noticed under the liver capsule (numerous in the PGP, few in the DGP) and around of the metastatic area (in the RGP). A granular cytoplasmic with membrane enhancement pattern was found only in the DGP, and together with nuclear expression in RGP and PGP (with highest intensity in the peripheral cells of metastatic area). A significant relation between CLIC 1 score in primary tumors and in LM (p=0.036) was found. It was identified an interrelation between RGP and CLIC 1 in metastasis but not in primary tumors. A similar correlation was found between CLIC 1 score in colorectal primary tumor and corresponding LM (p=0,039). CLIC 1 score in primary colorectal tumor was significant correlated to HGP (pushing and replacement), p=0.05.


Our data support CLIC 1 expression and HGP as important indicators of clinical evolution, mainly in the PGP and RGP of liver metastasis with digestive origin.

Clinical trial identification

Editorial acknowledgement

Legal entity responsible for the study

\"Victor Babeş” University of Medicine and Pharmacy, Timişoara, Romania.


Has not received any funding.


All authors have declared no conflicts of interest.

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