Abstract 46P
Background
Malignant ascites is one of the main complications in patients with peritoneal carcinomatosis. In present study we apply perfusion procedure with sodium bicarbonate solution in mice with Erlich ascites carcinoma.
Methods
ICR (CD-1) mice were intraperitoneal injected with 6 x 10ˆ5 Ehrlich carcinoma cells suspended in 0.2 ml of PBS solution. At day 7 after tumor injection mice were intraperitoneal perfused with 0.9 % sodium chloride solution (SC) in group pH0 (n=14) or with 1 % sodium bicarbonate solution containing 0.9 % sodium chloride (SB) in group pH+ (n=14). Mice in control group (n=14) were intact. The perfusion procedure consisted of tumor ascites evacuation, double intraperitoneal administration of 10 ml SC or SB with 10 minutes incubation followed by evacuation, intraperitoneal washing with 0.9 % SC. Cell concentration in ascites and pH were measured.
Results
The ascites pH values were 6.9 +/- 0.1 in all groups. Cell concentrations at day of perfusion, on the 7th and 11th days after were around 100, 200 and 300 x 10ˆ6 cells per ml respectively in all groups but ascites volume was visually lower in pH+ group. Mice had median survival of 24 days in pH+ group, which was significantly different from median survival of 17 days in group pH0 and control (p<0.05).
Conclusions
Sodium bicarbonate is effective treatment of mice with Erlich ascites carcinoma. We may suggest that intraperitoneal perfusion with sodium bicarbonate solution could be effective alternative to paracentesis or addition to HIPEC in palliative care of malignant ascites.
Clinical trial identification
Legal entity responsible for the study
The authors.
Funding
Has not received any funding.
Disclosure
All authors have declared no conflicts of interest.