787P - Endotoxicosis and properties of erythrocyte membranes in patients with cervical cancer receiving combination therapy with dendritic cell-based immunotherapy

Background

The efficacy of antitumor treatment depends on the function of proteins and structural components of cells. We studied the effect of immunotherapy with dendritic cell vaccine (DCV) as a part of combination therapy on the functional status of albumin, endogenous intoxication and structural and functional properties of erythrocyte membranes.

Methods

The patients with cervical cancer (CC) underwent neoadjuvant chemotherapy (CT) with cisplatin and bleomycin, followed by 2 cycles of DCV, for a total of 6 courses of CT and 12 cycles of DCV. Total and effective concentrations of albumin (TCA and ECA), reserve binding capacity of albumin (ABC), levels of medium mass molecules (MMM), toxicity index (TI=TCA/ECA–1) and the intoxication factor (IF=MMM₂₅₄/ECA•1000), as well as structural and functional parameters of erythrocyte membranes (by fluorimetry with the hydrophobic pyrene probe), were determined in the blood of 24 patients and in 20 women without cancer.

Results

Before the treatment, patients demonstrated the accumulation of MMM₂₅₄ and MMM₂₈₀ by 34.5% and 26.4% (p<0.003), and an increase in TI by 2.3 times (p=0.004), IF by 1.7 times (p=0.009). Even more pronounced changes were observed after CT: an increase in MMM₂₅₄ by 44.5% (p=0.0008), decrease in ECA and ABC by 23.9% (p<0.001), increase in TI by 3.4 times, IF by 1.8 times. Starting from the 4th DCV, the functional activity of albumin restored, normalization of MMM and the IF was observed, TI increased by 2.2 times relative to healthy women. An increased fluidity of membranes and an increase in the polarity of the lipid bilayer were revealed at the initial stages of the study, apparently associated with increased LPO processes in the blood of patients with CC initially and after CT. Multi-course vaccine therapy had a noticeable normalizing effect on all the studied parameters of the structural and functional state of erythrocyte membranes.

Conclusions

The results of the study indicate that the ability of albumin to control endotoxicosis, as well as changes in the structural and, therefore, functional properties of membranes can be used as prognostic markers of successful antitumor therapy.

Clinical trial identification

Editorial acknowledgement

Legal entity responsible for the study

The authors.

Funding

Has not received any funding.

Disclosure

All authors have declared no conflicts of interest.