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Poster Display session 1

2728 - MicroRNA expression and DNA methylation profiles do not distinguish between primary and recurrent well-differentiated liposarcoma

Date

28 Sep 2019

Session

Poster Display session 1

Topics

Tumour Site

Sarcoma

Presenters

Melissa Vos

Citation

Annals of Oncology (2019) 30 (suppl_5): v683-v709. 10.1093/annonc/mdz283

Authors

M. Vos1, R. Boers2, A.L.M. Vriends3, J. Boers2, P.F. van Kuijk3, W. Van Houdt4, G.J.L.H. van Leenders5, M. Wagrodzki6, W.F.J. van IJcken7, J. Gribnau2, D.J. Grünhagen8, C. Verhoef8, S. Sleijfer9, E.A.C. Wiemer10

Author affiliations

  • 1 Medical Oncology And Surgical Oncology, Erasmus MC Cancer Institute, 3015GD - Rotterdam/NL
  • 2 Developmental Biology, Erasmus MC, 3015 GD - Rotterdam/NL
  • 3 Medical Oncology, Erasmus MC Cancer Institute, 3015GD - Rotterdam/NL
  • 4 Surgical Oncology, Netherlands Cancer Institute/Antoni van Leeuwenhoek hospital (NKI-AVL), 1066 CX - Amsterdam/NL
  • 5 Pathology, Erasmus MC, 3015 CN - Rotterdam/NL
  • 6 Pathology, Maria Skłodowska-Curie Institute-Oncology Center, 02-781 - Warsaw/PL
  • 7 Center For Biomics, Erasmus MC, 3015 GD - Rotterdam/NL
  • 8 Surgical Oncology, Erasmus MC Cancer Institute, 3015 GD - Rotterdam/NL
  • 9 Medical Oncology, Erasmus MC Daniel den Hoed Cancer Center, 3015 GD - Rotterdam/NL
  • 10 Medical Oncology, Erasmus MC Cancer Institute, 3015CN - Rotterdam/NL
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Resources

Abstract 2728

Background

Approximately one-third of the patients with well-differentiated liposarcoma (WDLPS) will develop a local recurrence after resection of the primary tumor. Not much is known about the molecular relationship between the primary tumor and the recurrent tumor, which is important to reveal potential drivers of recurrence. Here we investigated the biology of recurrent WDLPS by comparing paired primary and recurrent WDLPS using microRNA profiling and genome-wide DNA methylation analyses.

Methods

Paired primary and recurrent WDLPS formalin-fixed and paraffin-embedded tumor samples were collected (N = 27 pairs). MicroRNA expression profiles were determined using TaqMan® Low Density Array (TLDA) cards, capable of detecting 754 microRNAs. Genome-wide DNA methylation and differentially methylated regions were assessed by methylated DNA sequencing (MeD-seq).

Results

A supervised cluster analysis based on differentially expressed microRNAs between paired primary and recurrent WDLPS did not reveal a clear cluster pattern separating the primary from the recurrent tumors. The clustering was also not based on tumor localization, time to recurrence, age or the status of the resection margins. Changes in DNA methylation between primary and recurrent tumors were extremely variable, and no consistent DNA methylation changes were found. As a result, a supervised clustering analysis based on differentially methylated regions between primary and recurrent tumors did not show a distinct cluster pattern based on any of the features. Subgroup analysis for tumors localized in the extremity or the retroperitoneum also did not yield a clear distinction between primary and recurrent WDLPS samples.

Conclusions

Primary and recurrent WDLPS could not be distinguished based on microRNA expression and DNA methylation profiles and no common microRNA and DNA methylation alterations for recurrence could be identified.

Clinical trial identification

Editorial acknowledgement

Legal entity responsible for the study

The authors.

Funding

Has not received any funding.

Disclosure

All authors have declared no conflicts of interest.

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