21P - Hsa_circ_0009061 inhibits the progression of bladder cancer through the miR-889-3p/CPEB3 axis

Background

The malignant progression of bladder cancer is a key influencing factor for its poor prognosis. In recent years, numerous studies have shown that circular RNA (circRNA) can regulate the expression of genes in multi-levels, which may play a key role in malignant tumors.

Methods

This study applied bioinformatics methods to preliminarily screened the differentially expressed circular RNAs between bladder cancer and corresponding adjacent tissue. Real-time quantitative PCR(RT-qPCR) experiments were performed to identify differentially expressed circular RNAs in cell lines and tissues of bladder cancer. The functional experiments including plate cloning, CCK-8, Edu and xenograft tumor models were implemented to investigate the biological behavior changes of bladder cancer induced by knocking down hsa_circ_0009061 in vitro and in vivo. Bioinformatics methods were applied to predict miRNAs that circular RNA might bind to and the miRNA target genes, and the real-time quantitative PCR, RNA pulldown,dual luciferase reporter experiment and western blot were utilized to explore the mechanism of hsa_circ_0009061 regulating bladder cancer progression.

Results

The bioinformatics analysis and RT-qPCR find that hsa_circ_0009061 was downregulated in cell lines and tissues of bladder cancer. After successfully knocking down hsa_circ_0009061 in bladder tumor cells, functional experiments including plate cloning, Edu and CCK-8 revealed that silencing hsa_circ_0009061 could promote the malignant proliferation of bladder tumor cell lines in vitro. Xenograft tumor modelalso verified that silencing hsa_circ_0009061 could promote tumor formation in vivo. Mechanistic studies have shown that hsa_circ_0009061 can act as a competitive endogenous RNA to bind to hsa-miR-889-3p and regulate the expression of CPEB3, resulting in the malignant progression of bladder cancer.

Conclusions

This study found that hsa_circ_0009061 was downregulated in cell lines and tumor specimens of bladder cancer and could affect the progression of bladder cancer. Mechanistically, hsa_circ_0009061 can bind to hsa-miR-889-3p to regulate the expression of CPEB3, suggesting that hsa_circ_0009061 may be a new treatment option for bladder cancer.

Editorial acknowledgement

Clinical trial identification

Legal entity responsible for the study

The authors.

Funding

Has not received any funding.

Disclosure

All authors have declared no conflicts of interest.