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Poster Display session

98P - Potential predictive biomarkers for response to immunotherapy in breast angiosarcoma

Date

15 Mar 2024

Session

Poster Display session

Presenters

Annabella Di Mauro

Citation

Annals of Oncology (2024) 9 (suppl_2): 1-32. 10.1016/esmoop/esmoop102441

Authors

A. Di Mauro1, G. Scognamiglio1, M. Cerrone1, R.A. Rega1, O. Clemente2, C. Mignogna3, L. Cannella2, A. Pizzolorusso2, F. Picozzi2, M. Cantile4, P. Moccia1, D. Iervolino1, A. De Chiara3, G. Ferrara1, S. Tafuto2

Author affiliations

  • 1 Unit Patology, Istituto Nazionale Tumori IRCCS - Fondazione G. Pascale, 80049 - Somma Vesuviana/IT
  • 2 Rare Tumors And Sarcomas Dept., Istituto Nazionale Tumori IRCCS - Fondazione G. Pascale, 80131 - Napoli/IT
  • 3 Histopathology Of Lymphomas And Sarcomas Ssd, Istituto Nazionale Tumori IRCCS - Fondazione G. Pascale, 80049 - Somma Vesuviana/IT
  • 4 Scientific Directorate, Istituto Nazionale Tumori IRCCS - Fondazione G. Pascale, 80131 - Napoli/IT

Resources

This content is available to ESMO members and event participants.

Abstract 98P

Background

Angiosarcoma is a relatively rare neoplasm of the breast (less than 0.05% of all malignant mammary tumours). Tumours can arise : i) de novo; secondary to a long standing lymphoedema ; iii) secondary to radiation therapy. In the last few years, the importance of studies about tumour microenvironment (TME) has been steadily growing, especially in order to identify potential responders to immune checkpoint inhibitors (ICIs) even in angiosarcoma. The aim of this study was to evaluate some TME features in angiosarcoma of the breast and to correlate such features with clinicopathological and follow up data.

Methods

The retrospective study was performed on tissue samples with medical records available at The Istituto Nazionale Tumori ‘G. Pascale’, Naples, Italy. Immunohistochemical parameters evaluated encompassed lymphoid cell markers (CD3+, CD8+, GrzB+, FoxP3+, CD103+) and immune checkpoint receptors (PD-L1, VISTA, TIM3, OX40). Fluorescence in situ hybridization was used to assess c-myc amplification. The immunohistochemical expression of the mismatch repair proteins was also investigated and, in ‘indeterminate’ cases, followed by automated RT-qPCR examination for microsatellite instability (IdyllaTM platform). Overall survival (OS) was estimated by Kaplan-Meier method and compared with Log-rank test.

Results

Nine cases of primary (pAS) and nine cases of secondary angiosarcoma (sAS) were retrieved. Comparison of mean tumor-infiltrating lymphocytes (TILs) and immune checkpoint receptor (ICrs) densities between pAS and sAS showed a statistically significant difference only in GrzB+ cells, which were more represented in pAS (p=0.042). Furthermore, we simultaneously quantified the number of stained T cells by multiple immunofluorescence to verify the balance between cytotoxic (CT) and immunosuppressive (IS) activity in the TME. In about 50% of cases, we found colocalisation of CD8+ and FOXP3+ cells. Although not significant, a higher degree of immunosuppression was observed in SAS cases (p = 0.266).

Conclusions

Our prelimary data suggest that sAS discloses an immunosuppressive environment; therefore, compared to pAS, sAS might be a better candidate to immunotherapy.

Clinical trial identification

Editorial acknowledgement

Legal entity responsible for the study

The authors.

Funding

Has not received any funding.

Disclosure

All authors have declared no conflicts of interest.

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