Abstract 118P
Background
To assess the relationship between the morphological characteristics of primary choroidal melanoma and survival rates.
Methods
The study was performed at the material of 74 patients diagnosed with choroidal melanoma cT1-4, for wich enucleation was the primary treatment. The immunohistochemical staining of tumor samples using antibodies to CD3, CD68, CD34 was performed. Double staining of CD34 + CD3 and CD34 + CD68 was carried out in order to analyze the location of immune cells relative to the microvessels. The regression analysis method was used to evaluate the relationship between histological characteristics, microvascular pattern, number of CD3- and CD68-positive cells, immune cells location pattern and survival rates.
Results
Two groups of microvascular patterns were distinguished: linear (“straight”, “parallel”, “parallel with intersections”) and arcuate (“arches”, “arches with branching”, “loops” and “network”). We also identified 3 variants of the location of CD68-positive cells relative to the microvessels: perivascular - cells are localized mainly near the tumor vessels, combined - cells are evenly distributed between the paravascular compartment and the compartment of tumor cells and diffuse - CD68-positive cells are located in mostly among tumor cells. Statistically significant relationship with survival rates was found for the pT category and the CD3- and CD68-positive cells ratio. With an increase in category T and a CD3/CD8>1 ratio, the risk of disease progression increased by 1.5 and 3.7 times, respectively. Median progression-free survival for CD3/CD68 >1 was 34 (95% CI 24-117) months. With a CD3/CD68 ratio <1, the median was not reached (p=0.001). Median adjusted survival for CD3/CD68 >1 was 43 (95% CI 30-100) months. With a CD3/CD68 ratio <1, the median was not reached (p=0.014).
Conclusions
The ratio of CD3- and CD68-positive cells has a statistically significant effect on adjusted survival and progression-free survival in patients with choroidal melanoma. With a CD3/CD8>1 ratio, the risk of disease progression increased by 3.7 times (p=0.012), the risk of death from choroidal melanoma increased by 2 times (p=0.01).
Clinical trial identification
Editorial acknowledgement
Legal entity responsible for the study
The authors.
Funding
Has not received any funding.
Disclosure
All authors have declared no conflicts of interest.