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Poster session 09

78P - Peroxiredoxin-1 knockout negatively affects the viability of ph+ B-cell acute lymphoblastic leukemia cells and sensitizes them to tyrosine kinase inhibitors

Date

21 Oct 2023

Session

Poster session 09

Topics

Cancer Biology;  Molecular Oncology

Tumour Site

Leukaemias

Presenters

Jaromir Hunia

Citation

Annals of Oncology (2023) 34 (suppl_2): S187-S214. 10.1016/S0923-7534(23)01931-2

Authors

J. Hunia1, Ł. Komorowski1, K. Szczygieł2, M. Firczuk1

Author affiliations

  • 1 Department Of Immunology, Medical University of Warsaw, 02-097 - Warsaw/PL
  • 2 Sa, Polpharma Biologics, 80-172 - Gdansk/PL

Resources

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Abstract 78P

Background

Breakpoint cluster region-Abelson (BCR:ABL1) gene fusion is an essential oncogene in both chronic myeloid leukemia (CML) and Philadelphia-positive (Ph+) B-cell acute lymphoblastic leukemia (B-ALL). While tyrosine kinase inhibitors (TKIs) are effective in up to 95% of CML patients, 50% of Ph+ B-ALL cases do not respond to treatment or relapse. This calls for new therapeutic approaches for Ph+ B-ALL. Dysregulation of redox homeostasis and reliance on the thioredoxin (TXN) antioxidant system has been previously shown in B-ALL. Herein, we investigated the role of peroxiredoxin-1 (PRDX1), an enzyme responsible for the scavenging of hydrogen peroxide, in Ph+ myeloid and lymphoid leukemia.

Methods

PRDX1 knockout and apoptotic proteins were assessed by immunoblotting. The numbers of viable BV173 (lymphoid) and K562 (myeloid) cells were evaluated by light microscope counting after discriminating dead cells with Trypan Blue staining. Cell cycle and proliferation were assessed with MUSE and Click-iT EdU tests, respectively. The viability of K562 and BV173 knockouts treated with TKIs was assessed with propidium iodide staining and flow cytometry.

Results

PRDX1 knockout reduced the numbers of viable BV173 cells grown in vitro, but did not affect the numbers of K562 cells. The addition of hydrogen peroxide scavenges to the culture medium restored the viability of BV173 cells. We observed no significant differences in cell proliferation or in cell cycle fractions in BV173sgPRDX1 cells. Notably, in contrast to the myeloid K562 cell line, lymphoid B-ALL cells lacking PRDX1 were much more sensitive to three tested TKIs: imatinib, dasatinib, and ponatinib.

Conclusions

The results reveal that the important role of PRDX1 for Ph+ leukemic cell viability and sensitivity to TKIs is restricted to lymphoid leukemias. The observed reduced numbers of PRDX1 knockout cells were not the effect of an altered cell cycle or proliferation but most likely due to increased apoptosis. These findings are part of a larger ongoing in vitro and in vivo study examining the sensitization of Ph+ lymphoid leukemia to TKIs through inhibition of the thioredoxin system and PRDX1.

Clinical trial identification

Editorial acknowledgement

Legal entity responsible for the study

Medical University of Warsaw.

Funding

SONATA BIS 2015/18/E/NZ5/00723 grant funded by the National Science Centre, entitled “Novel pro-oxidative strategies in the treatment of B cell acute lymphoblastic leukemia”.

Disclosure

All authors have declared no conflicts of interest.

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