Abstract 21P
Background
Pathogenic mutations in the epigenetic modulator histone-lysine N-methyltransferase 2C (KMT2C) occur in up to 1 in every 6 breast cancers and KMT2C likely functions as a tumour suppressor. Despite this high frequency, therapeutic implications of deleterious KMT2C mutations are poorly understood and detailed pre-clinical work is still needed. DNA damage repair pathways may be affected by KMT2C mutations according to published preclinical data. The aim of this study is to identify the effects of KMT2C knockdown in vitro in breast cancer cells as a basis for identifying potential synthetic lethality drug targets.
Methods
MCF-7 (ER+ breast cancer) cells were cultured and 500,000 cells per well were plated on 6-well plates. KMT2C siRNA was transfected via Lipofectamine as per protocol in three wells, with universal control siRNA in three wells. Total RNA was extracted and sent for sequencing. RNA expression was quantified by RT-qPCR.
Results
RNA-seq confirmed reduction in expression of KMT2C in KMT2C siRNA-treated cells vs controls. RT-qPCR showed a reduction in expression of DNA damage repair genes including BRCA1 (RQ = 0.36), BRCA2 (RQ = 0.37), RAD51 (RQ = 0.36), RAD54L (RQ = 0.28) and POLD3 (RQ = 0.47).
Conclusions
KMT2C loss-of-function in breast cancer may impact DNA damage response pathways including homologous recombination and therefore be implicated in response to established treatments such as PARP-inhibitors. Further study including mapping of co-dependencies by whole genome siRNA screens in KMT2C-mutant breast cancer cells to identify synthetic lethality targets, as well as cell toxicity assays, are being performed in light of these results.
Clinical trial identification
Editorial acknowledgement
Legal entity responsible for the study
Beaumont RCSI Cancer Centre.
Funding
Has not received any funding.
Disclosure
All authors have declared no conflicts of interest.
Resources from the same session
68P - Dendritic polylysine with paclitaxel and triptolide codelivery for enhanced NSCLC ferroptosis through the accumulation of ROS
Presenter: Huae Xu
Session: Poster session 09
69P - Novel monoclonal antibodies can distinguish Cripto-1 from Cripto-3 proteins: Clinical implications and potential new biomarkers
Presenter: Josune Garcia-Sanmartin
Session: Poster session 09
70P - The human intratumor mycobiome is significantly influenced by an individual's race
Presenter: Dan Coster
Session: Poster session 09
71P - Preclinical characterization of ARX305: A next-generation anti-CD70 antibody drug conjugate for the treatment of CD70-expressing cancers
Presenter: Lillian Skidmore
Session: Poster session 09
72P - Impact of extended panel of genes for germline cancer testing
Presenter: Shaheenah Dawood
Session: Poster session 09
73P - Preclinical and clinical presentation of the nerve-driven tumor spread
Presenter: Dawid Sigorski
Session: Poster session 09
74P - Characterization of ERBB2 variation and their association with immune response in solid tumours
Presenter: Dong Wang
Session: Poster session 09
75P - Double-stranded RNA transfection induced anti-tumour effect mediated by dual RIG-I and TLR-3 immune pathways
Presenter: Jiayu Tai
Session: Poster session 09
76P - Improvement of whole-cell cancer vaccine anti-tumor effect by different injection methods
Presenter: Chin yang Chang
Session: Poster session 09
77P - Normative data on the sexual health questionnaires - QLQ-SHQ22, and the sexual domains of the QLQ-BR23/BR45 - for Norwegian general population with and without cancer
Presenter: Ragnhild Åsberg
Session: Poster session 09