Abstract 31P
Background
Breast cancer tissues include carcinoma cells and stromal cells, and intra-tumoral stroma that consists of different types of cells. For this point, cell-cell interaction and communication have a potential role in cancer progression. Mono-cell culture is used for cancer treatment approaches. However, cell-cell interaction and communication can not be evaluated on mono-culture cells. So, co-culture models provide low-cost screening to determine cell proliferation for drug application before moving forward to in vivo models. Also, determination of cell morphology in co-culture system is critical to understand advantages.
Methods
In our previous study, novel targeting therapeutic effects of specific micelles including Doxurobicin-loading and unused specific HER-2 peptide sequence (VSSTQDFP) were investigated. The cytotoxicity of micelles is determined on mono-culture as well as the co-culture system of SKBR-3- HER2-(+) and chronic myeloid leukemia cancer cells (K562-HER2-(-)) that constituted an important and first method for drug selectivity studies. During this co-culture study, we observed the differentiation morphology of K562 cells. Following this observation, cell morphology analysis was done on GFP-transfected K562 cells by using the Image J program in terms of size and shape.
Results
In our last study, targeted micelles had more cytotoxicity on SKBR-3 cells to compare co-cultured K562 cells. After this study, the cell differentiation of K562 cells (GFP transfected) was observed after microscopy observation by fluorescence microscopy. After the co-culture of transfected K562 cells and SKBR-3 cells at 24-hours, more than half of K562 cells gained cell adhesion properties. Also, cell morphology in terms of size distribution and shape is different on K562 cells because of microvilli-like structures.
Conclusions
Co-culture studies support novel and well-defined approaches in drug development and determination of targeted drug efficiency. Tumor microenvironment maintains a cell-cell interaction that has a critical role in the initiation and progression of cancer formation. In our future perspective, the cell adhesion molecule, and cytokine signals will be determined to understand cell invasion, migration, and metastasis.
Clinical trial identification
Editorial acknowledgement
This study was supported by the research grants from The Scientific and Technological Research Council of Turkey, “Development of Breast Cancer Targeted, More Stable, IV Double-Moiety pH-Sensitive, Drug-Conjugated, Multifunctional Micelle-Based Nanocarriers and Investigation of Their in vitro Efficiency”, Project Number: 116R057
Legal entity responsible for the study
Prof. Dr. Yusuf Baran.
Funding
Research grants from the Scientific and Technological Research Council of Turkey, “Development of Breast Cancer Targeted, More Stable, IV Double-Moiety pH-Sensitive, Drug-Conjugated, Multifunctional Micelle-Based Nanocarriers and Investigation of Their in vitro Efficiency”, Project Number: 116R057.
Disclosure
All authors have declared no conflicts of interest.