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Poster Display session 1

5287 - Use of droplet digital polymerase chain reaction for detecting minimal residual disease: a prospective, multi-institutional study

Date

28 Sep 2019

Session

Poster Display session 1

Topics

Translational Research

Tumour Site

Presenters

Hyunkyung Park

Citation

Annals of Oncology (2019) 30 (suppl_5): v435-v448. 10.1093/annonc/mdz251

Authors

H. Park1, D. Shin2, I. Kim2, S. Sohn3, Y. Koh2, J. Lee4, K. Lee4, D. Kim4, H. Kim5, J. Ahn5, J. Lee6, S. Bang6, J. Cheong7, S. Park8, S. Park9, Y.J. Lee10, S. Ahn5

Author affiliations

  • 1 Internal Medicine, Boramae Medical Center, 22332 - Seoul/KR
  • 2 Internal Medicine, Seoul National University Hospital, 03080 - Seoul/KR
  • 3 Internal Medicine, Kyungpook National University Hospital, 41944 - Daegu/KR
  • 4 Internal Medicine, Asan Medical Center, Seoul/KR
  • 5 Internal Medicine, Chonnam National University, Hwasun Hospital, Hwasun/KR
  • 6 Internal Medicine, Seoul National University, Bundang Hospital, Seongnam/KR
  • 7 Internal Medicine, Yonsei University, Severance Hospital, Seoul/KR
  • 8 Internal Medicine, Chosun University Hospital, Gwangju/KR
  • 9 Internal Medicine, Haeundae Paik Hospital, Busan/KR
  • 10 Internal Medicine, Kyungpook National University Hospital, Daegu/KR

Resources

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Abstract 5287

Background

Droplet digital polymerase chain reaction (ddPCR) is an exact method of measurement in cancer research. The aim of this prospective study was to evaluate minimal residual disease (MRD) using ddPCR in patients with chronic myeloid leukemia (CML). We also evaluated treatment outcome depending on BCR/ABL1 transcript level using ddPCR.

Methods

Between May 2013 and November 2014, CML patients treated with nilotinib as the first-line therapy were enrolled. BCR/ABL1 transcripts levels were evaluated using ddPCR at the first time of complete molecular response (CMR). CMR was verified by quantitative real-time polymerase chain reaction.

Results

We enrolled 15 patients from 7 institutions. The treatment period and median follow-up period were 45 months (range, 37–55 months) and 47 months (range, 39–61 months), respectively. The median value of BCR/ABL1 measured by ddPCR was 3.6 copies/20 μl (range, 1.2–6.8 copies/20 μl). Patients with a high level of BCR/ABL1 transcript had a greater tendency to lose the CMR during the follow-up period (0/10 (0%) for low levels vs. 2/5 (40%) for high levels, P = 0.095). In addition, patients with a low level of BCR/ABL1 transcript showed a longer duration of CMR than those with a high level (rate of sustained CMR at 2 years: 100% for low levels vs. 37.5% for high levels, P = 0.032).

Conclusions

We found that ddPCR is a sensitive method for detecting MRD and that MRD could affect the duration of the treatment response.

Clinical trial identification

Editorial acknowledgement

Legal entity responsible for the study

The authors.

Funding

Novartis Pharmaceuticals Corporation.

Disclosure

All authors have declared no conflicts of interest.

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