Abstract 4293
Background
The purpose of the study was to assess the significance of the local cytokine composition in patients with esophageal squamous cell carcinoma (EC) for the disease prognosis.
Methods
Cytokines were studied in tumor, peritumoral (PT) and resection line (RL) tissues of 36 patients with stage II-III EC. All patients received surgery; further treatment depended on the tumor stage. During the observation, patients were divided into groups with short and prolonged progression-free survival (< and >1.5 years). Levels of TNF-α, IL-1b, IL-6, IL-8, and IL-10 were determined in tissue homogenates by ELISA and calculated in pg/mL/g of protein. Discriminant and ROC analyses were performed with the calculation of the area under a ROC curve (AUC) and cut-off points for statistically significant indicators sharing with maximum diagnostic sensitivity (DSe) and specificity (DSp) two alternative signs: the presence or absence of the risk of early progression.
Results
Patients showed different initial levels of tissue pro-and anti-inflammatory cytokines. PT of patients with the further progression was characterized by higher levels of TNF-α (by 1.9) and IL-10 (by 1.6 times), and their tumor tissues – by 2.1 times higher IL-1b and 1.6 times higher IL-8 (p < 0.05). The ROC analysis demonstrated the following cut-off values: in tumors 57.5 pg/mL/g (IL-1b) and 4.5 pg/mL/g (IL-10), in PT 36.0 pg/mL/g (IL-8) and 7.4 pg/mL/g (TNF-α), in RL 9.8 pg/mL/g (TNF-α) and 5.2 pg/mL/g (IL-10). Exceeding them involved the risk of early disease progression. Levels of TNF-α in PT, IL-10 in tumors and RL showed good (AUC 0.8-0.9), and levels of TNF-α in RL, IL-1b in tumors and IL-8 in PT - satisfactory (AUC 0.7-0.8) ability to recognize the risk of early EC progression, p < 0.005.
Conclusions
Evaluation of local levels of cytokines allowed identifying the risk criteria for the development of early progression of EC, which along with clinical signs (the process spread) determine its prognosis.
Clinical trial identification
Editorial acknowledgement
Legal entity responsible for the study
The authors.
Funding
Has not received any funding.
Disclosure
All authors have declared no conflicts of interest.
Resources from the same session
5694 - Findings from a new specialist remote Counselling Service for Neuroendocrine Neoplasm (NEN) patients and family members
Presenter: Catherine Bouvier
Session: Poster Display session 2
Resources:
Abstract
4725 - Hematologic malignancies in temozolomide-treated metastatic pancreatic neuroendocrine tumors
Presenter: Nicole Balmaceda
Session: Poster Display session 2
Resources:
Abstract
5842 - Efficacy and toxicity of combination chemotherapy with cyclophosphamide, vincristine and an anthracycline in patients with metastatic extrapulmonary neuroendocrine carcinoma
Presenter: Leonidas Apostolidis
Session: Poster Display session 2
Resources:
Abstract
1543 - An Australian multi-centre experience of the use of peptide receptor radionuclide therapy for bronchial carcinoid tumours.
Presenter: Lisi Lim
Session: Poster Display session 2
Resources:
Abstract
4175 - Extra-pulmonary (EP) high grade (HG) neuroendocrine carcinoma (NEC): real-life outcomes of fifty-eight patients from a Portuguese cancer center.
Presenter: Rita Conde
Session: Poster Display session 2
Resources:
Abstract
3274 - Efficacy of immune check-point inhibitors (ICPi) in large cell neuroendocrine tumors of lung (LCNET)
Presenter: Shira Sherman
Session: Poster Display session 2
Resources:
Abstract
3534 - HORMONET: Study of Tamoxifen in Well Differentiated Neuroendocrine Tumors and Hormone Receptor Positive Expression
Presenter: Milton Barros
Session: Poster Display session 2
Resources:
Abstract
2137 - Clinical utility of Metabolic Tumor Volume in Papillary Thyroid Carcinoma
Presenter: Norihiko Takemoto
Session: Poster Display session 2
Resources:
Abstract
3864 - Correlation of thyroglobulin (Tg) oscillations with progression-free survival (PFS) in patients with radioactive iodine-refractory (RAI-R) differentiated thyroid carcinoma (DTC) treated with multikinase inhibitors (MKI).
Presenter: Jorge Hernando Cubero
Session: Poster Display session 2
Resources:
Abstract
2820 - Analytical validation of a thyroid cancer diagnostic method based on the relative quantification of CLDN10, HMGA2 and LAMB3 expression
Presenter: Mateus Barrosfilho
Session: Poster Display session 2
Resources:
Abstract