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Poster Discussion 1 – Immunotherapy of cancer

2234 - A phase 1 trial of GEN-009, a neoantigen vaccine using ATLAS™, an autologous immune assay, to identify immunogenic and inhibitory tumor mutations.

Date

28 Sep 2019

Session

Poster Discussion 1 – Immunotherapy of cancer

Presenters

Przemyslaw Twardowski

Citation

Annals of Oncology (2019) 30 (suppl_5): v475-v532. 10.1093/annonc/mdz253

Authors

P. Twardowski1, M.L. Johnson2, M. Stein3, U. Vaishampayan4, M.L. Gillison5, L. McNeil6, L. Dowal6, D. DeOliveira7, M. Jain7, J. Price8, R. Hernandez8, A. DeCillis8, N. Singh7, T. Davis8, J. Flechtner6, R.B. Cohen9

Author affiliations

  • 1 Medical Oncology, City of Hope, 91010 - Duarte/US
  • 2 Medical Oncology, Sarah Cannon Research Institute-Cancer Centre, 37203 - Nashville/US
  • 3 Hematology/oncology, Columbia University Medical Center, 10032 - New York/US
  • 4 Oncology, Barbara Ann Karmanos Cancer Institute, 48201 - Detroit/US
  • 5 Head & Neck Medical Oncology, MD Anderson Cancer Center, 77030 - Houston/US
  • 6 Research, Genocea Biosciences, 02140 - Cambridge/US
  • 7 Pharmaceutical Sciences, Genocea Biosciences, 02140 - Cambridge/US
  • 8 Clinical Operations, Genocea Biosciences, 02140 - Cambridge/US
  • 9 Center For Head And Neck Cancer, Abramson Cancer Center, PA 19104 - Philadelphia/US

Resources

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Abstract 2234

Background

Tumor-specific neoantigens provide personalized targets for immunotherapy. Vaccines against epitopes predicted by in silico approaches very rarely induce CD4+ and CD8+ ex vivo T cell responses regardless of formulation. ATLAS selects neoantigens for vaccine inclusion using ex vivo screening of all patient-specific mutations to identify pre-existing CD4+ or CD8+ T cell responses and to exclude inhibitory peptides that suppress immunity and accelerate tumor progression. Preliminary data suggest that the inhibitory peptide profile may predict tumor response to immunotherapy.

Methods

GEN-009-101 is a phase I/IIa study testing safety, immunogenicity and clinical activity in immune responsive tumors. After next-generation tumor sequencing and ATLAS testing of autologous leukocytes, up to 20 stimulatory synthetic long peptides adjuvanted with poly-ICLC make each personalized vaccine. The immunogenicity pilot has enrolled 9 patients in remission to receive GEN-009 monotherapy.

Results

4 patients have participated to the primary immunogenicity readout at day 50 (some data pending). The 17 doses given across patients have induced only mild local discomfort and no DLT. ATLAS results show high interpatient variability. Vaccination has generated immune responses against 94% of administered peptides, and both CD8+ and CD4+ responses in a 44 hr ex vivo fluorospot assay. Ten-day in vitro stimulation (IVS) assays result in broader immune responses.Table:

1178PD

PTTumor TypeSomatic Mutations/MbATLAS NeoantigensPost-vaccination Response*
StimulatoryInhibitoryex vivo CD4/CD8IVS CD4/CD8Total Positive
1NSCLC1.256010% / 40%100% / 20%100%
2Bladder3.1516450% / 38%63% / 50%88%
3Melanoma28.69199416% / 38%75% / TBDTBD
4Bladder3.53181TBDTBDTBD
5NSCLC3.5616955% / 45%TBDTBD
*

number of positive peptides/total peptides immunized

Conclusions

GEN-009 is a neoantigen vaccine that identifies tumor specific immune targets from the individual patient’s repertoire. Immunogenicity data show that ATLAS can, with very high frequency, identify relevant neoantigens and exclude suppressive peptides. Clinical vaccination with PD-1 blockade is in process.

Clinical trial identification

NCT03633110, issued 6-Aug-2018.

Editorial acknowledgement

Legal entity responsible for the study

Genocea Biosciences.

Funding

Genocea Biosciences.

Disclosure

P. Twardowski: Advisory / Consultancy, Speaker Bureau / Expert testimony: Bayer; Speaker Bureau / Expert testimony: Janssen; Speaker Bureau / Expert testimony: Pfizer; Speaker Bureau / Expert testimony: Astellas; Speaker Bureau / Expert testimony: Sanofi; Advisory / Consultancy: Bristol-Myers Squibb. M.L. Johnson: Advisory / Consultancy, Research grant / Funding (institution), Travel / Accommodation / Expenses: Genentech / Roche; Advisory / Consultancy: Celgene; Advisory / Consultancy, Research grant / Funding (institution), Travel / Accommodation / Expenses: Boehringer Ingelheim; Advisory / Consultancy, Research grant / Funding (institution): Sanofi; Advisory / Consultancy, Research grant / Funding (institution): Mirati; Advisory / Consultancy, Research grant / Funding (institution): LOXO; Advisory / Consultancy: Calithera; Advisory / Consultancy, Research grant / Funding (institution), Travel / Accommodation / Expenses: AstraZeneca; Advisory / Consultancy, Research grant / Funding (institution), Travel / Accommodation / Expenses: Merck; Advisory / Consultancy: Araxes Pharma; Advisory / Consultancy: Mersana Therapeutics ; Advisory / Consultancy, Research grant / Funding (institution): BeiGene; Advisory / Consultancy, Research grant / Funding (institution), Travel / Accommodation / Expenses: Incyte; Advisory / Consultancy, Research grant / Funding (institution), Travel / Accommodation / Expenses: Pfizer; Advisory / Consultancy, Research grant / Funding (institution): Guardant Health; Advisory / Consultancy, Research grant / Funding (institution), Travel / Accommodation / Expenses: Bristol-Myers Squibb; Advisory / Consultancy: Ribon Therapeutics ; Research grant / Funding (institution), Travel / Accommodation / Expenses: AbbVie; Travel / Accommodation / Expenses: Astellas; Research grant / Funding (institution), Travel / Accommodation / Expenses, Spouse / Financial dependant: Clovis; Spouse / Financial dependant: Otsuka Pharmaceuticals; Research grant / Funding (institution): BerGenBio; Research grant / Funding (institution): Lilly; Research grant / Funding (institution), Travel / Accommodation / Expenses: EMD Serono; Research grant / Funding (institution): Janssen; Research grant / Funding (institution): Genmab; Research grant / Funding (institution): Stemcentrix; Research grant / Funding (institution): Novartis; Research grant / Funding (institution): Checkpoint Therapeutics; Research grant / Funding (institution): Array Biopharma; Research grant / Funding (institution): Regeneron; Research grant / Funding (institution): Apexigen; Research grant / Funding (institution): Tarveda; Research grant / Funding (institution): Adaptimmune; Research grant / Funding (institution): Syndax; Research grant / Funding (institution): Neovia; Research grant / Funding (institution): Hengrui Therapeutics, INC; Research grant / Funding (institution), Travel / Accommodation / Expenses: Daiichi - Sankyo; Research grant / Funding (institution): Lycera; Research grant / Funding (institution): G1 Therapeutics; Research grant / Funding (institution): Dynavax; Research grant / Funding (institution): Cytomx; Research grant / Funding (institution): Birdie; Research grant / Funding (institution): Corvus; Research grant / Funding (institution): Genocea; Research grant / Funding (institution): Gritstone; Research grant / Funding (institution): Amgen; Research grant / Funding (institution): Kadmon; Research grant / Funding (institution): Acerta; Research grant / Funding (institution): Takeda; Research grant / Funding (institution): Shattuck Labs; Research grant / Funding (institution): GlaxoSmithKline; Travel / Accommodation / Expenses: Exelixis; Travel / Accommodation / Expenses: Sysmex Inostics; Travel / Accommodation / Expenses: Vapotherm. M. Stein: Advisory / Consultancy, Research grant / Funding (institution): Merck Sharp & Dohme; Advisory / Consultancy: Exelixis; Research grant / Funding (institution): Oncoceutics; Research grant / Funding (institution): Janssen Oncology; Research grant / Funding (institution): Medivation/Astellas; Research grant / Funding (institution): Advaxis; Research grant / Funding (institution): Suzhou Kintor Pharmaceuticals; Research grant / Funding (institution): Harpoon; Research grant / Funding (institution): Bristol-Myers Squibb; Research grant / Funding (institution): Genocea; Research grant / Funding (institution): Lilly; Research grant / Funding (institution): Nektar; Research grant / Funding (institution): Seattle Genetics; Research grant / Funding (institution): Xencor. M.L. Gillison: Advisory / Consultancy: Amgen; Advisory / Consultancy: AstraZeneca; Advisory / Consultancy: Aspyrian; Advisory / Consultancy: Celgene; Advisory / Consultancy: Britsol-Myers Squibb; Advisory / Consultancy: GlaxoSmithKline; Advisory / Consultancy: Genocea; Advisory / Consultancy: Lilly; Advisory / Consultancy: NewLink; Advisory / Consultancy: Bayer; Advisory / Consultancy: Roche; Advisory / Consultancy: Genentech; Advisory / Consultancy: Ventana; Advisory / Consultancy: Biomimetix. L. McNeil: Shareholder / Stockholder / Stock options, Full / Part-time employment: Genocea. L. Dowal: Shareholder / Stockholder / Stock options, Full / Part-time employment: Genocea. D. DeOliveira: Shareholder / Stockholder / Stock options, Full / Part-time employment: Genocea. M. Jain: Shareholder / Stockholder / Stock options, Full / Part-time employment: Genocea. J. Price: Shareholder / Stockholder / Stock options, Full / Part-time employment: Genocea. R. Hernandez: Leadership role, Shareholder / Stockholder / Stock options, Full / Part-time employment: Genocea. A. DeCillis: Advisory / Consultancy: Genocea; Advisory / Consultancy, Shareholder / Stockholder / Stock options: Exelixis; Advisory / Consultancy: Pyramid; Advisory / Consultancy: Daiichi Sankyo. N. Singh: Leadership role, Shareholder / Stockholder / Stock options, Full / Part-time employment, Officer / Board of Directors: Genocea. T. Davis: Leadership role, Shareholder / Stockholder / Stock options, Full / Part-time employment, Officer / Board of Directors: Genocea. J. Flechtner: Leadership role, Shareholder / Stockholder / Stock options, Full / Part-time employment, Officer / Board of Directors: Genocea. R.B. Cohen: Advisory / Consultancy, Research grant / Funding (institution): Genocea; Advisory / Consultancy, Research grant / Funding (institution): Innate Biopharma; Advisory / Consultancy, Research grant / Funding (institution): HEAT Biologics; Advisory / Consultancy: Cantargia; Advisory / Consultancy: Tmunity; Research grant / Funding (institution): Arrys; Research grant / Funding (institution): Celldex. All other authors have declared no conflicts of interest.

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