Abstract 106P
Background
Polyps are considered as obligate precancerous disease. Different authors consider that percent of malignization ranges from 20% in single polyps to 95% in diffuse polyposis. Current research examines the results of the early diagnosis of colon cancer by using the biomarker CDKN2A for development of molecular-biological methods of prognostication.
Methods
Investigations were carried out in 30 patients during the period 2017-2019, the study included patients with single polyps, multiple polyps, polyposis of the colon, as well as patients without a history of neither familial nor sporadic cancer. 21 patients had polyposis, in 9 single colon polyps. The men were 19 women 11. The average age of patients was 52,4 ± 3,8 years. The levels of methylation of the gene CDKN2A were determined 18 patients. The material for the isolation of DNA was a serum obtained from venous blood and and biopsy material in amount of 50 mg. DNA extraction was held according to standards. After the conversion of DNA methyl-specific PCR was held.
Results
Among 30 patients with polyps and polyposis of the colon serum gene CDKN2A methylated DNA was detected in 18 patients. In 4 patients malignization of the polyp was determined. The volume of surgical intervention in patients with malignization was the same as cancer. It patients without malignization surgical intervention was limited with resection of the intestine without lymph dessection. In 8 patients the test result was negative. We did not perform surgical intervention to these patients they were sent to dynamic observation. Thus, the diagnostic efficacy in detection colorectal cancer in early diagnosis of by means of methylated DNA of the gene CDKN2A was - 60.1%.
Conclusions
If there is a positive test for methylation of the CDKN2A-gene, the risk of colorectal cancer is considered very high and these patients underwent surgical treatment. Application of molecular biological marker CDKN2A may be an effective method in early diagnosis of malignization of polyps. This method of diagnosis requires a more in-depth study, based on a study of a larger number of observations. Further investigations of the CDKN2a gene in ulcerative colitis, Krohn's disease, Lynch syndrome, Jeghers syndrome might be continued.
Clinical trial identification
Editorial acknowledgement
Legal entity responsible for the study
NCC of Uzbekistan.
Funding
Has not received any funding.
Disclosure
All authors have declared no conflicts of interest.
Resources from the same session
41P - Clinical verification on the relationship between serum lipid metabolism and the immune microenvironment in breast cancer patients
Presenter: Wataru Goto
Session: Poster display session
Resources:
Abstract
42P - Genome wide copy number analysis of circulating tumour cells in breast cancer liver metastasis
Presenter: Saber Imani
Session: Poster display session
Resources:
Abstract
43P - A hotspot variants p.H1047R and p.H1047L in p110α/ΔNp63α complex affects structure, function and contributes to susceptibility metastatic breast cancer
Presenter: Zou Linglin
Session: Poster display session
Resources:
Abstract
44P - Correlation of circulating tumour cells with PET-CT in metastatic breast cancer
Presenter: Venkata Pradeep Babu Koyyala
Session: Poster display session
Resources:
Abstract
45P - The challenge of evaluating new targeted therapies: Opportunities in stratifying the therapeutic response per tumour location
Presenter: Hubert Beaumont
Session: Poster display session
Resources:
Abstract
46P - Plasma soluble CD36 of breast cancer based on pathological and clinical characteristics
Presenter: Aditia Romadhoni
Session: Poster display session
Resources:
Abstract
47P - Investigation of the use of a novel S-1 administration method for treating metastatic and recurrent breast cancer
Presenter: MAYUKO MIKI
Session: Poster display session
Resources:
Abstract
48P - Development of MDA-MB-231-3D-Spheroid as a reliable model for studying Nav1.5 and nNav1.5-mediated breast cancer metastasis
Presenter: Ahmad Murtadha
Session: Poster display session
Resources:
Abstract
49P - Biochemical study on modifying role of variants of leptin gene and its receptor on serum leptin levels in breast cancer
Presenter: Alshimaa Alhanafy
Session: Poster display session
Resources:
Abstract
50P - Prognostic factors of recurrence or distant metastasis in elderly breast cancer patients
Presenter: Seungju Lee
Session: Poster display session
Resources:
Abstract