467P - The free-circulating mtDNA copies number in plasma of patients with NSCLC

Background

Lung cancer is a serious problem and one of the most common cancer diseases worldwide. Many studies were shown that free-circulating mtDNA levels are increased in the serum/plasma of patients with various malignancies. Here we analysed mtDNA levels in the plasma of patients with NSCLC compared to the health individuals.

Methods

68 patients with NSCLC and 59 healthy individuals were recruited for this study. A quantitative analysis of the free-circulating mtDNA was conducted using the qPCR. To create a standard curve, the purified PCR product of the 490 bp mtDNA fragment was used. The DNA concentration determined using the PicoGreen dsDNA Quantification Kit. The copy number of the free-circulating mtDNA was calculated according to the protocol of Applied Biosystems.

Results

The levels of free-circulating mtDNA in patients with NSCLC were found in range from 2.7x10¹⁰ to 6,51x10¹⁴ with a mean value 3,04x10¹³ copies/μL. In plasma samples of healthy subjects the levels of mtDNA copies were found to range from 6,0x10⁹ to 1,73x10¹³ with a mean value 7,46x10¹¹. Thus, the levels of free-circulating mtDNA in patients with NSCLC were higher in 6.6 times than in those of healthy subjects (P < 0.05).

Conclusions

Results of this study suggest that high free-circulating mtDNA copies number is a promising marker for diagnosis and prediction of non-small cell lung cancer.

Clinical trial identification

Editorial acknowledgement

Legal entity responsible for the study

Authors.

Funding

Ministry of Education and Science of the Republic of Kazakhstan (grant no. 1258/GF4).

Disclosure

All authors have declared no conflicts of interest.