Abstract 255P
Background
MEX3A is an evolutionarily conserved RNA-binding protein (RBP), of which functions and target genes in mammals remain largely unknown. Also, no previous study has shown the role of MEX3A in endometrial cancer.
Methods
We screened the differentially expressed genes (DEGs) and miRNAs (DEmiRs) between endometrial cancer samples and paired adjacent noncancerous endometrium samples with RNA-seq data. Then, we used package clusterProfiler to profile the GO and KEGG enrichment analysis of the most highly correlated genes with MEX3A, as well as the overlap genes with up-regulated DEGs. We evaluated the prognostic effect of genes using the Kaplan-Meier Plotter database. MEX3A tissue-specific expression was analyzed via the Human Protein Atlas database and TIMER. Methylation analysis was generated by MethHC and Wanderer.
Results
The volcano plot of DEmiRs showed a significant reduction in tumor suppressor miR-139 in tumor tissues compared to normal tissues. We totally identified 422 target genes of miR-139-5p using the prediction software TargetScan (7.1 version). MEX3A was one of the seven overlapping genes that we got between the miRNA target genes and the 842 up-regulated DEGs. MEX3A was significantly overexpressed in many types of tumor tissues, and interestingly, it had the highest expression in the female tissues. In addition, high MEX3A expression was significantly correlated with poor OS and progression-free survival (PFS) in endometrial cancers (OS HR = 2.07, P = 0.0021; PFS HR = 2.11, P = 0.0047). The GO analysis of top 5% most highly correlated genes with MEX3A in endometrial cancer revealed that biology processes associated with RNA splicing were mainly enriched. Simultaneously, the most enriched pathway was spliceosome, which indicated that the RBP may play an important role in the RNA splicing. Meanwhile, the MEX3A promoter region in the tumor tissue was hypomethylated compared to normal tissue. In addition, the MEX3A promotor differential methylation level is negatively correlated with MEX3A mRNA expression (corr = -0.512).
Conclusions
These findings suggest MEX3A is a potential prognostic biomarker in endometrial cancer and might play an oncogenic role as a novel cancer-critical splicing factor.
Clinical trial identification
Editorial acknowledgement
Legal entity responsible for the study
The author.
Funding
Has not received any funding.
The author has declared no conflicts of interest.
Resources from the same session
448P - Incidence and severity of chemotherapy induced peripheral neuropathy in geriatric population who are receiving chemotherapy
Presenter: Alekhya Mandepudi
Session: Poster display session
Resources:
Abstract
449P - Utility of screening tool for assessment of chemotherapy induced peripheral neuropathy in cancer patients
Presenter: PRUTHVI Paibhavi
Session: Poster display session
Resources:
Abstract
450P - Supportive care to control nausea and dizziness in malignant tumours: A systematic review
Presenter: Rainer Spiegel
Session: Poster display session
Resources:
Abstract
451P - The safety of ramucirumab without H1-antihistamines as a premedication in patients with solid cancers: A retrospective study
Presenter: Nobuhiro Hattori
Session: Poster display session
Resources:
Abstract
452P - Expression of erythropoietin receptor in patient with anemia related chemotherapy and its correlation with absolute reticulocyte count
Presenter: Irza Wahid
Session: Poster display session
Resources:
Abstract
453P - Body mass index did not correlate with short term overall survival in breast cancers
Presenter: Jungsun Lee
Session: Poster display session
Resources:
Abstract
454P - Predictors of severity and comparison of CTCAE V3.0 vs V4.3 in assessing chemotherapy induced extravasation injuries
Presenter: Sangana Reddy
Session: Poster display session
Resources:
Abstract
455P - Assessment of nutritional status and quality of life among cancer patients undergoing chemotherapy
Presenter: Sri Prathima
Session: Poster display session
Resources:
Abstract
456P - Use of gamma irradiated bone allografts in treatment of various bone tumours
Presenter: Anchal Mishra
Session: Poster display session
Resources:
Abstract
457P - The prevalence of vitamin D deficiency in Thai cancer patients, its dynamics and association with cancer survival
Presenter: Chavapon Ngokngarm
Session: Poster display session
Resources:
Abstract