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Poster Display session 3

3594 - Tumor mutation burden (TMB), PD-L1, IFN-γ signaling identify subgroups of patients (pts) who benefit from durvalumab (D, anti-PDL1) or D and tremelimumab (T, anti-CTLA4) treatment in urothelial bladder cancer (UC)

Date

30 Sep 2019

Session

Poster Display session 3

Presenters

Christophe Massard

Citation

Annals of Oncology (2019) 30 (suppl_5): v475-v532. 10.1093/annonc/mdz253

Authors

C. Massard1, H. Si2, Q. Zhang3, B. Higgs3, R. Raja4, S.E. Abdullah5, A. Gupta5, W. Li6, M.S. Van der Heijden7

Author affiliations

  • 1 Ditep, Gustave Roussy - Cancer Campus, 94805 - Villejuif/FR
  • 2 Oncology Research, AstraZeneca, 20878 - Gaithersburg/US
  • 3 Translational Bioinformatics, AstraZeneca, 20878 - Gaithersburg/US
  • 4 Translational Medicine Oncology And Pharmacogenics, AstraZeneca, 20878 - Gaithersburg/US
  • 5 Clinical Development, AstraZeneca, 20878 - Gaithersburg/US
  • 6 Translational Sciences, AstraZeneca, 20878 - Gaithersburg/US
  • 7 Urogenital Carcinogenesis, Netherlands Cancer Institute (NKI), 1066 - Amsterdam/NL
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Resources

Abstract 3594

Background

We conducted NGS of baseline UC tumors to elucidate molecular predictors of clinical outcomes on D/D+T.

Methods

CP1108/NCT01693562 was a nonrandomized phase I/II trial including 201 2L UC pts who were treated with D (10 mg/kg Q2W). Study 10/NCT02261220 was a phase I trial including 190 2L UC pts treated with D (20 mg/kg Q4W) +T (1 mg/kg Q4W). Pts with ≥25% PD-L1 expression in tumor or immune cells were scored as PD-L1+ by IHC. RNA-seq (n = 62) and whole exome sequencing (WES; n = 37) were used in CP1108. The IFN-γ gene signature (IFNGS) was calculated as described (Higgs B et al., 2018). TMB was calculated as somatic coding mutation count per mega base from WES data with TMB≥top tertile as TMB high. FMI assay (n = 62) was conducted in Study 10 with TMB≥top tertile as TMB high. ctDNA dynamics using Guardant360 were calculated using an established method (Raja R, et al., 2018). Wilcoxon, log-rank tests and COX-PH model were used in relevant statistical tests.

Results

Among ITT pts, 55% and 44.5% pts were PD-L1+ in CP1108 and Study 10 respectively. PD-L1 status correlated with IFNGS (p < 0.001), whereas PD-L1 did not correlate with TMB. PD-L1+ correlated with better OS in CP1108 (HR = 0.46, p < 0.001) but not in Study 10 (HR = 0.75, p = 0.2). IFNGS significantly correlated with better PFS in CP1108 (HR = 0.5, p = 0.02). Higher TMB in CP1108 using the median cutoff trended towards better OS (HR = 0.51, p = 0.18), but no clear trend using the top tertile cutoff due to small sample size. Higher TMB in Study 10 correlated with improved OS (HR = 0.34, p = 0.01). In Study 10, TMB and PD-L1 double positive pts (n = 14, 24%) had best OS and pts low in both had worst (n = 19, 32%, HR = 0.23, p = 0.01). Clearance of ctDNA after treatment correlated with better OS (HR = 0.23, p = 0.006) and PFS (HR = 0.33, p = 0.01) in combined UC pts from the 2 studies.

Conclusions

Both PD-L1 and TMB predict for survival benefit in UC pts treated with D/D+T. They identify overlapping but generally distinct patient populations. Double highs perform the best. ctDNA loss following treatment can be predictive for better survival.

Clinical trial identification

NCT01693562; NCT02261220.

Editorial acknowledgement

Legal entity responsible for the study

AstraZeneca.

Funding

AstraZeneca.

Disclosure

C. Massard: Advisory / Consultancy: Amgen; Advisory / Consultancy: Astellas; Advisory / Consultancy: AstraZeneca; Advisory / Consultancy: Bayer; Advisory / Consultancy: Celgene; Advisory / Consultancy: Genentech; Advisory / Consultancy: Ipsen; Advisory / Consultancy: Novartis; Advisory / Consultancy: Roche; Speaker Bureau / Expert testimony: Sanofi; Advisory / Consultancy: Janssen; Advisory / Consultancy: Lilly; Advisory / Consultancy: Pfizer; Advisory / Consultancy: Orion. H. Si: Full / Part-time employment: AstraZeneca. Q. Zhang: Full / Part-time employment: AstraZeneca. B. Higgs: Shareholder / Stockholder / Stock options, Full / Part-time employment: AstraZeneca. R. Raja: Shareholder / Stockholder / Stock options, Full / Part-time employment: AstraZeneca. S.E. Abdullah: Travel / Accommodation / Expenses, Shareholder / Stockholder / Stock options, Full / Part-time employment: AstraZeneca. A. Gupta: Shareholder / Stockholder / Stock options, Full / Part-time employment: AstraZeneca; Shareholder / Stockholder / Stock options, Licensing / Royalties, Use of immunotherapy in the treatment of cancer-patent: Bristol-Myers Squibb. W. Li: Shareholder / Stockholder / Stock options, Full / Part-time employment: AstraZeneca. M. van der Heijden: Advisory / Consultancy: AstraZeneca; Advisory / Consultancy, Research grant / Funding (institution): BMS; Advisory / Consultancy: MSD; Advisory / Consultancy, Research grant / Funding (institution): Roche/Genentech; Advisory / Consultancy, Research grant / Funding (institution): Astellas; Advisory / Consultancy: Seattle Genetics.

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