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Advantages

RT-PCR has high sensitivity and specificity for a known fusion transcript [1]. In addition, the methodology is relatively fast and low cost [2]. 

References

  1. Argani P, Fritsch M, Kadkol SS et al. Detection of the ETV6-NTRK3 chimeric RNA of infantile fibrosarcoma/cellular congenital mesoblastic nephroma in paraffin-embedded tissue: application to challenging pediatric renal stromal tumors. Mod Pathol 2000; 13: 29-36.
  2. Xu T, Wang H, Huang X et al. Gene Fusion in Malignant Glioma: An Emerging Target for Next-Generation Personalized Treatment. Transl Oncol 2018; 11: 609-618.

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