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Advantages

The advantages of DNA-based NGS are those common to all NGS techniques as well as those specific to the DNA-based NGS. The advantages of DNA-based NGS are summarised in the following table.

Table 21: Advantages of DNA-based NGS for Testing for NTRK Gene Fusions

Advantages

  • Assesses molecular information such as mutations, copy number changes, and microsatellite instability status [1] with high resolution [2].
  • Detects rearrangements in multiple genes and introns [3, 4].
  • Can identify translocation partners without direct targeting of these partners [2].

References

  1. Cocco E, Scaltriti M, Drilon A. NTRK fusion-positive cancers and TRK inhibitor therapy. Nat Rev Clin Oncol 2018; 15: 731-747.
  2. Abel HJ, Al-Kateb H, Cottrell CE et al. Detection of gene rearrangements in targeted clinical next-generation sequencing. J Mol Diagn 2014; 16: 405-417.
  3. Zehir A, Benayed R, Shah RH et al. Mutational landscape of metastatic cancer revealed from prospective clinical sequencing of 10,000 patients. Nat Med 2017; 23: 703-713.
  4. Beadling C, Wald AI, Warrick A et al. A Multiplexed Amplicon Approach for Detecting Gene Fusions by Next-Generation Sequencing. J Mol Diagn 2016; 18: 165-175.

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