Abstract 75P
Background
Marine macroalgae Sargassum wightii is abundantly distributed on the southern coasts of Tamil Nadu, India. S. wightii was less explored for its medicinal properties. Although selenium nanoparticles (SeNPs) have been regarded as safer and efficient to enhance various biological functions, the agglomeration of SeNPs hinder its biological applicability. Hence, in the present study, SeNPs was surface functionalized with biologically active compounds present in S. wightii to produce SW-SeNPs which will not only enhance its biological availability but also will synergistically enhance its therapeutic property.
Methods
The biosynthesized SW-SeNPs was characterized using Transmission Electron Microscopy (TEM), Zeta potential analysis, Fourier Transform-Infrared spectroscopy (FT-IR) and X-ray diffraction (XRD). The anticancer effect of SW-SeNPs against A549 cells was determined. Various parameters such as cell viability, reactive oxygen species (ROS) production, DNA fragmentation, cell cycle progression and apoptosis were studied. Also, the level of p53, p-Akt and caspase-3 were determined using flow cytometry.
Results
SW-SeNPs was found to be in the range of 20 - 40 nm size with crystalline nature. The negative zeta potential of SW-SeNPs and FT-IR analysis confirmed the surface binding of bioactive components present in S. wightii in the SW-SeNPs. SW-SeNPs was studied for its anticancer potential against A549 cells. SW-SeNPs affected the A549 cells viability in dose-dependent manner. SW-SeNPs treatment increased the ROS generation and led to DNA fragmentation in A549 cells. Also, the cells were arrested in G2/M phase which ultimately induced apoptosis of the A549 cells through caspase-3 activation. The molecular mechanism of SW-SeNPs-induced A549 cells apoptosis was found to be through overproduced ROS-mediated activation of p53 and AKT signal pathways.
Conclusions
Altogether, the present study demonstrated the anticancer therapeutic effect of SW-SeNPs against lung cancer.
Legal entity responsible for the study
The authors.
Funding
Has not received any funding.
Disclosure
All authors have declared no conflicts of interest.