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e-Poster Display Session

20P - Quantitative whole slide assessment of CD8+ tumour-infiltrating lymphocytes in small cell esophageal carcinoma in relation to clinical outcome

Date

09 Dec 2020

Session

e-Poster Display Session

Presenters

Zhihui Zhang

Citation

Annals of Oncology (2020) 31 (suppl_7): S1417-S1424. 10.1016/annonc/annonc389

Authors

Z. Zhang1, C. Zhang2, G. Zhang2, L. Xue2, Q. Zeng1

Author affiliations

  • 1 National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing/CN
  • 2 Chinese Academy of Medical Sciences and Peking Union Medical College - National Cancer Center, Cancer Hospital, Beijing/CN
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Abstract 20P

Background

Primary small cell esophageal carcinoma (SCEC) represents a rapidly progressive, aggressive, and rare malignancy without any established treatment strategy. Given the great success of cancer immunotherapy targeting the PD-1/PD-L1 pathway in small cell lung cancer and the central role of CD8+ tumor-infiltrating lymphocytes (TIL) in the response to immune checkpoint inhibitors, we set out to assess the expression pattern and clinical significance of CD8+ TIL in SCESs for the first time.

Methods

Totally, 147 SCEC patients underwent surgery at our institution between 1985 and 2019 were collected. The formalin-fixed paraffin-embedded whole tumor sections were immunostained for CD8 with monoclonal mouse antibody. Whole-slide scanning was performed and the tumor regions were annotated by a trained gastrointestinal pathologist. The digital image analysis was then performed to quantify the CD8+ TILs density. Baseline clinicopathological features and outcome data (relapse free survival (RFS) and overall survival (OS)) were correlated with CD8+ TIL states.

Results

The median number of CD8+ TIL cells in SCECs was 111.1 cells/mm2, with the range of 7.5 to 1458.6 cells/mm2. The mean destiny (195.9 cells/mm2) was used as cut-off value stratifying CD8+ TIL positive or negative groups. Positive CD8+ TILs infiltration was found significantly related to macroscopic tumor type (P=0.006), T stage (P=0.007) and TNM stage (P=0.005). Kaplan–Meier analysis showed that patients with positive CD8+ TILs state showed dramatically better RFS (HR = 0.456, 95% CI 0.309–0.674, P=0.0004) and OS (HR = 0.423, 95% CI 0.273–0.657, P=0.0008). Univariate and multivariate Cox regression analyses confirmed that CD8+ TIL state was the significant independent predictor for both RFS and OS, with P values of 0.0140 and 0.0457, respectively.

Conclusions

Here, for the first, we demonstrated that CD8+ TIL destiny was associated with an early stage and favorable clinical outcome in SCECs, supporting its role as a prognostic biomarker. These findings will not only help to improve risk-adapted therapeutic strategies, but also provide a rational basis for further investigation of PD-L1/PD-1-based immunotherapy for patients with SCECs.

Legal entity responsible for the study

The authors.

Funding

Has not received any funding.

Disclosure

All authors have declared no conflicts of interest.

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