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Poster Display session

386 - Relation between IDH1 status, histologic grade, immune-cell infiltration and expression of immune-related genes in patients with gliomas


14 Dec 2018


Poster Display session


Santiago Cabezas-Camarero


Annals of Oncology (2018) 29 (suppl_10): x1-x10. 10.1093/annonc/mdy493


S. Cabezas-Camarero1, R. Pérez-Alfayate1, I. Casado Fariñas1, M. Sáiz-Pardo Sanz1, I. Subhi-Issa1, P. Pérez-Segura1, V. García-Barberán2

Author affiliations

  • 1 Medical Oncology, Hospital Clinico Universitario San Carlos, 28040 - Madrid/ES
  • 2 Molecular Oncology Laboratory, Hospital Clinico Universitario San Carlos, 28040 - Madrid/ES


Abstract 386


Very few data exist regarding the immune profile of gliomas. Our aim was to study different immunological parameters according to IDH1 mutational status and histologic grade.


Patients with grade II to IV gliomas were prospectively enrolled. Immunohistochemistry (IHC) was used to quantify CD4+ and CD8+ T cells and to study IDH1 and PDL1 (> 5%) expression on tumor cells. These parameters were correlated to mRNA expression of 20 immune-related genes (CSF1R, CTLA4, CXCL9, CXCL10, CXCL13, FOXP3, GZMA, HLADRA, IDO1, INFG, PD1, PDL1, PDL2, PRF1, STAT1, STAT3, TIGIT, TIM3, VISTA, TLR7) according to IDH1 status and histologic grade.


Between February 2017 and July 2018, 20 patients (pts) were enrolled, 14 glioblastomas (GB: 9 IDHWT, 5 IDH1MUT) and 6 lower-grade gliomas (LGG: 2 IDHWT, 4 IDHMUT). Among 18 pts: PDL1 IHC was positive (PDL1IHC+) in 10 GB (3 IDH1MUT) and 1 LGG (IDH1MUT); CD4+ T cell infiltration (intratumoral (IT)/perivascular(PV)) in 5 GB (2 IDH1MUT); CD8+ T cell infiltration in 12 GB (8 IT+PV, 3 IT, 1 PV) and 5 LGG (1 IT, 4 IT+PV). PDL1IHC+ more frequent in GB + LGGIDH1wt vs LGGIDH1mut (P = 0.06). PDL1IHC+ tumors shoed lower expression of STAT1 (P = 0.042), TIM3 (P = 0.02) and TLR7 (P = 0.06). Tumors with > 20 CD8+ T cells/HPF showed higher expression of PD1 (P = 0.03), PRF1 (P = 0.03) and STAT1 (P = 0.03).


Histologic grade and IDH1 status identify glioma groups with different immunophenotypes. This results should be confirmed in a larger sample.

Editorial acknowledgement

Clinical trial identification

Legal entity responsible for the study

Santiago Cabezas.


GEINO (Grupo Español de Investigación en Neuro-Oncología).


All authors have declared no conflicts of interest.

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