Oops, you're using an old version of your browser so some of the features on this page may not be displaying properly.

MINIMAL Requirements: Google Chrome 24+Mozilla Firefox 20+Internet Explorer 11Opera 15–18Apple Safari 7SeaMonkey 2.15-2.23

ePoster Display

46P - Intraperitoneal perfusion with sodium bicarbonate solution can significantly increase the lifespan of mice with Ehrlich ascites carcinoma

Date

16 Sep 2021

Session

ePoster Display

Presenters

Alexey Bogdanov

Citation

Annals of Oncology (2021) 32 (suppl_5): S361-S375. 10.1016/annonc/annonc684

Authors

A.A. Bogdanov1, N.A. Verlov1, N.A. Knyazev1, V.V. Klimenko1, A.A. Bogdanov1, V.M. Moiseyenko2

Author affiliations

  • 1 Scientific Department, St. Petersburg Clinical Scientific and Practical Center for Specialised Types of Medical Care (Oncological), 197758 - St Petersburg/RU
  • 2 Administration, St. Petersburg Clinical Scientific and Practical Center for Specialised Types of Medical Care (Oncological), 197758 - St Petersburg/RU
More

Abstract 46P

Background

Malignant ascites is one of the main complications in patients with peritoneal carcinomatosis. In present study we apply perfusion procedure with sodium bicarbonate solution in mice with Erlich ascites carcinoma.

Methods

ICR (CD-1) mice were intraperitoneal injected with 6 x 10ˆ5 Ehrlich carcinoma cells suspended in 0.2 ml of PBS solution. At day 7 after tumor injection mice were intraperitoneal perfused with 0.9 % sodium chloride solution (SC) in group pH0 (n=14) or with 1 % sodium bicarbonate solution containing 0.9 % sodium chloride (SB) in group pH+ (n=14). Mice in control group (n=14) were intact. The perfusion procedure consisted of tumor ascites evacuation, double intraperitoneal administration of 10 ml SC or SB with 10 minutes incubation followed by evacuation, intraperitoneal washing with 0.9 % SC. Cell concentration in ascites and pH were measured.

Results

The ascites pH values were 6.9 +/- 0.1 in all groups. Cell concentrations at day of perfusion, on the 7th and 11th days after were around 100, 200 and 300 x 10ˆ6 cells per ml respectively in all groups but ascites volume was visually lower in pH+ group. Mice had median survival of 24 days in pH+ group, which was significantly different from median survival of 17 days in group pH0 and control (p<0.05).

Conclusions

Sodium bicarbonate is effective treatment of mice with Erlich ascites carcinoma. We may suggest that intraperitoneal perfusion with sodium bicarbonate solution could be effective alternative to paracentesis or addition to HIPEC in palliative care of malignant ascites.

Clinical trial identification

Legal entity responsible for the study

The authors.

Funding

Has not received any funding.

Disclosure

All authors have declared no conflicts of interest.

This site uses cookies. Some of these cookies are essential, while others help us improve your experience by providing insights into how the site is being used.

For more detailed information on the cookies we use, please check our Privacy Policy.

Customise settings
  • Necessary cookies enable core functionality. The website cannot function properly without these cookies, and you can only disable them by changing your browser preferences.