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Poster display - Cocktail

809 - Liquid Biopsy has a potential to predict the colorectal cancer patients with destiny for recurrence after curative surgery

Date

24 Nov 2018

Session

Poster display - Cocktail

Topics

Translational Research

Tumour Site

Colon and Rectal Cancer

Presenters

Toshiaki Toshima

Citation

Annals of Oncology (2018) 29 (suppl_9): ix28-ix45. 10.1093/annonc/mdy431

Authors

T. Toshima1, A. Nyuya2, U. Umeda1, K. Yasui1, K. Yoshida1, T. Fujiwara1, A. Goel3, T. Nagasaka2

Author affiliations

  • 1 Gastroenterological Surgery, Okayama University Hospital, 700-8558 - Okayama/JP
  • 2 Medical Oncology, Kawasaki Medical School Hospital, 701-0192 - Kurashiki/JP
  • 3 Center For Gastrointestinal Cancer Research; Center For Epigenetics, Cancer Prevention And Cancer Genomics, Baylor Research Institute And Charles A Sammons Cancer Center, Baylor University Medical Center, Gaston Street Dallas, TX/US
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Resources

Abstract 809

Background

Nowadays, detection of circulating cell-free tumor DNA (cfDNA) in serum is the frontier noninvasive approach. We reported the utility of detection of cancer-specific methylated CpGs from cfDNA for monitoring tumor dynamics during systemic chemotherapies in colorectal cancer (CRC) patients. The purpose of this study was to establish whether our assay can predict patients with risk for recurrence prospectively by analyzing blood obtained before curative surgery.

Methods

Methylation status of CpG sites, considered as cancer-specific alteration, and concentration of cfDNA were evaluated from blood plasma obtained before surgical resection. To analyze aberrant cancer-specific methylation, we modified the highly sensitive assay for bisulfite DNA followed by fluorescence-based PCR, as reported previously (Nagasaka T. et al, Analysis of fecal DNA methylation to detect gastrointestinal neoplasia, J Natl Cancer Inst. 2009 Sep 16;101(18):1244--58. doi: 10.1093/jnci/djp265. Epub 2009 Aug 21). This can detect 8 loci of target promoters, therefore the methylation score (MS) could be ranged from 0 to 8 at a given time, and we also evaluated methylation density in a quantitative manner.

Results

Of the 88 CRC patients enrolled, 16, 26, 25, and 21 patients were stage I, II, III, and IV, respectively. Therefore, to examine whether liquid biopsy can predict CRC patients with risk for recurrence, we focused on stage II and III patients. Of the 51 stage II and III patients, 5 patients received neoadjuvant therapies. Thus, a total of 46 stage II and III CRC patients were finally analyzed. Of the 46 CRC patients, during our observation period [median follow-up was 1056 days (range; 343-1401 days)], only three stage III patients experienced recurrences after curative surgery. Mean plasma MS of the patients with recurrence was increased compared with the patients without recurrences (1.11 vs. 0.8, not significant). When we assessed a methylation density, the mean plasma methylation density of the patients with recurrence was 16078 points (95%CI: 3074-29082) whereas that of the patients without recurrence was 6611 points (95%CI: 3704-9519, P = 0.16).

Conclusions

Although the number of the patients who experienced recurrences after curative resection was too small, our circulating cell-free DNA-based assay showed a potential to predict CRC patients with risk for recurrence after curative surgery.

Editorial acknowledgement

Clinical trial identification

Legal entity responsible for the study

Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences.

Funding

Has not received any funding.

Disclosure

All authors have declared no conflicts of interest.

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