Oops, you're using an old version of your browser so some of the features on this page may not be displaying properly.

MINIMAL Requirements: Google Chrome 24+Mozilla Firefox 20+Internet Explorer 11Opera 15–18Apple Safari 7SeaMonkey 2.15-2.23

Poster display - Cocktail

307 - Breast tumor associated exosomes mediate loss of antitumor immune response by arresting cytotoxic T cell functions in the tumor microenvironment

Date

24 Nov 2018

Session

Poster display - Cocktail

Presenters

Soumya Chatterjee

Citation

Annals of Oncology (2018) 29 (suppl_9): ix8-ix12. 10.1093/annonc/mdy427

Authors

S. Chatterjee1, A. Chatterjee2, S. Jana2, H. Roy3, N. Nargis2, A. Bhattacharyya2

Author affiliations

  • 1 Department Of Zoology, University of Calcutta, 700019 - Kolkata/IN
  • 2 Department Of Zoology, University of Calcutta, 700019 - kolkata/IN
  • 3 Department Of Surgery, Calcutta Medical College, 700073 - kolkata/IN
More

Resources

Abstract 307

Background

Inactivity of cytotoxic T lymphocytes (CTLs) in the tumor microenvironment allows an aggressive progression of breast cancer (BC). Multiple cells and factors have been reported to contribute to the arrest of the antitumor response of cytotoxic T lymphocytes (CTL) in the tumor microenvironment. Tumor-derived exosomes (TDEs) play a key role in tumor-host crosstalk and that exosome secretion, composition, and functional capacity are altered as tumors progress. How these TDEs regulate CTL function is still not clear. This study addresses a mechanism that TDEs follow to modulate CTL activity in the tumor microenvironment.

Methods

Breast cancer patient serum, MDA-MB 231 cell derived exosomes were characterized by Dynamic Light Scattering (DLS) and western blot analysis. Sorted CD8+ T cells from PBMC of healthy volunteers were activated with CD3/CD28, recombinant IL2 and treated with TDEs for different timespans. Exosome uptake by T cells was studied by confocal microscopy. Cell proliferation and differentiation was studied by flow cytometry analysis. qPCR analysis was performed to study the genes related to CD8+ naïve T cell differentiation and proliferation. Cytokine profile and T-bet expression were analysed by qPCR and flow cytometry analysis.

Results

Activated CTLs uptake TDEs in a time-dependent manner. TDEs reduced cellular proliferation of activated CTLs and blocked effector differentiation. Cytotoxic properties of CTLs were attenuated post uptake of TDE. The later was found to alter T-bet expression in the CD8+ T cells.

Conclusions

TDEs modulate CD8+T cell proliferation and differentiation in the tumor micro-environment. They also reduce the cytotoxic properties of CD8+T lymphocytes. TDE-mediated dysfunction of CTLs in the tumor microenvironment leads to proliferation of breast cancer cells.

Editorial acknowledgement

Clinical trial identification

Legal entity responsible for the study

Arindam Bhattacharyya, Professor, Department of Zoology, University of Calcutta.

Funding

Has not received any funding.

Disclosure

All authors have declared no conflicts of interest.

This site uses cookies. Some of these cookies are essential, while others help us improve your experience by providing insights into how the site is being used.

For more detailed information on the cookies we use, please check our Privacy Policy.

Customise settings