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Poster lunch

1525 - Effects of Sinomenine, Cepharanthine, and Tetrandrine on 2D and 3D Cultured Triple Negative Breast Cancer Cells. (140P)


18 Nov 2017


Poster lunch


Clinical Research;  Cancer Biology


Anna Kiyomi


Annals of Oncology (2017) 28 (suppl_10): x39-x41. 10.1093/annonc/mdx658


A. Kiyomi1, R. Miyakawa1, N. Uematsu2, H. Ono2, Y. Nakajima1, T. Hirano2, M. Sugiura1

Author affiliations

  • 1 Drug Safety & Risk Management, Tokyo University of Pharmacy & Life Sciences, 1920392 - Tokyo/JP
  • 2 Clinical Pharmacology, Tokyo University of Pharmacy & Life Sciences, 1920392 - Tokyo/JP


Abstract 1525


P-glycoprotein (P-gp) is known to contribute to chemotherapy resistance. Tetrandrine (TET) has been reported to inhibit P-gp function. Sinomenine (SIN) may have a similar efficacy, while its effect on P-gp have little been clarified. Cepharanthine (CEP), a structural TET analog, has little been investigated for its anti-cancer efficacy. 3-Dimensionally (3D) cultured spheroids reflect in vivo environment closer than 2-dimensionally (2D) cultured cells. Then, we aimed to investigate the suppressive efficacy of these herbal ingredients on the growth of 2D and 3D cultured triple negative breast cancer cells.


MDA-MB-231 cells were cultured in a well of normal flat bottom plate or DSeA-3D Plate filled with TGP. After incubating in 5% CO2 for one night, cells or spheroids were treated by alone/combination of doxorubicin (DOX), docetaxel (DOC), SIN, CEP, or TET and then further cultured for 48 or 72hs. After incubating, the cell viability in each well was evaluated by WST-8 assay. Likewise, cells or spheroids were treated by DOC and/or a P-gp inhibitor verapamil (Vera) and evaluated the cell viability.


DOX or DOC alone suppressed 2D cultured MDA-MB-231 cells dose-dependently, and significant effects were observed by > 5 × 10−7M DOX or > 2 × 10−8M DOC (p 


3D cultured TNBC cells showed chemotherapy resistance compared with 2D cultured cells, and it may not be because of P-gp. TET, SIN and CEP suppress the 2D cultured TNBC cells, and thus further studies are encouraging for their combined use with anti-cancer drugs.

Legal entity responsible for the study

Tokyo University of Pharmacy & Life Sciences

Clinical trial identification

Legal entity responsible for the study

Tokyo University of Pharmacy & Life Sciences




All authors have declared no conflicts of interest.

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