A tumor that starts from the mucus secreting glands of the colon or rectal region of large intestine is called as colorectal cancer. Primarily it spreads deeper into colon or rectum wall. In advance stages it proliferates to lymph nodes and other organs above 95% of colorectal cancers are adenocarcinomas.
Current research was conducted to determine the role of Wnt, FOXO and micro-RNAsignaling cascade in upregulation of CTNNB1 gene expression in colorectal cancer(CRC) patients. Quantitative real-time PCR was performed to determine the gene expression level and analysis.
Based on gene expression through qRT-PCR the expression levels of CTNNB1, TP-53, MiR-328, MiR-140, MiR-145, FOXO1 and FOXO3 were highly expressed in CRC patients in comparison to control tissue samples. Up regulation of CTNNB1 which is a transcriptional factor for many genes and is responsible for oncogenesis after mutation is also noted. Expression levels of MiR-328, MiR-140 and MiR-145 were highly expressed and revealed no involvement of MicroRNAs in current colorectal cancer cases and similarly the up regulation of FOXO1 and FOXO3 were revealed a clear evidence of their potential contribution in colorectal cancer and it’s metastasizing. The data was analyzed statistically by using ANOVA and DMR test for significance.
Mirco-RNA and Wnt signaling cascade up regulate CTNNB1 gene in colorectal carcinoma.
Clinical trial identification
Legal entity responsible for the study
Ethical Review Committee, Punjab Medical College, Faisalabad
All authors have declared no conflicts of interest.