Endocrine disrupting chemicals such as Bisphenol A (BPA) has the potential to cause adverse effects to health by disrupting normal human homeostasis. BPA is widely used plasticizer and it is an ideal polymer for epoxy resins and polycarbonates. Therefore, humans are regularly exposed to this chemical through ingestion of water, food and beverages which are contaminated with BPA. Studies have linked BPA exposure in humans with the risk of cardiovascular disease development, yet the direct effects of BPA on cardiomyocytes morphology have not been entirely explored. In here we aimed to investigate the cytotoxic effects of BPA on cells structure of isolated neonatal rat cardiomyocytes culture.
Cardiomyocytes were isolated from newborn Sprague Dawley rats. Cardiomyocytes were treated with 10-7 to 10-4 M of BPA and subjected for beating rates experiment, cell viability assay and Scanning Electron microscopy. In beating rate experiment, significant reduction in rates (50% -55%, ± 1.5275, p ≤ 0.05) were observed in cardiomyocytes treated with 10-7 to 10-4 M of BPA.
In beating rate experiment, significant reduction in rates (50% -55%, ± 1.5275, p ≤ 0.05) were observed in cardiomyocytes treated with 10−7 to 10−4 M of BPA. Interestingly, cell viability was markedly reduced (54%, ± 0.0026, p ≤ 0.05) in cardiomyocytes treated with 10−7 M of BPA compared to cells in untreated group and others BPA concentration. Cardiomyocytes show altered cell surface homogeneity after BPA exposure. The signs of flattening cardiomyocytes cell surface, reduction of the size, and blurring of the cell borders were observed and evident after exposure to 10−7 to 10−4 M of BPA.
This study provides in vitro evidence of the potential adverse effects of BPA on cardiomyocytes morphology. However, further investigation would be required to understand how BPA is likely to be potentially hazardous to heart and other heart cells.
Clinical trial identification
Legal entity responsible for the study
Universiti Teknologi MARA Animals Ethics Committee
All authors have declared no conflicts of interest.