Oops, you're using an old version of your browser so some of the features on this page may not be displaying properly.

MINIMAL Requirements: Google Chrome 24+Mozilla Firefox 20+Internet Explorer 11Opera 15–18Apple Safari 7SeaMonkey 2.15-2.23

Poster lunch

961 - Cell free microRNA‐200c expression as a prognostic/predictive biomarker in breast cancer patients. (40P)

Date

18 Nov 2017

Session

Poster lunch

Presenters

Mirza Masroor

Citation

Annals of Oncology (2017) 28 (suppl_10): x7-x15. 10.1093/annonc/mdx653

Authors

M. Masroor

Author affiliations

  • Biochemistry, Maulana Azad Medical College, 110002 - Delhi/IN
More

Resources

Abstract 961

Background

Breast cancer in women is an important cause of cancer‐related mortality. MicroRNA‐200c (miR‐200) is important in the epithelial to mesenchymal transition (EMT) and plays a major role in breast cancer metastasis. The present study focused on investigating the prognostic importance of cell-free miR‐200c expression in breast cancer patients.

Methods

A total of 75 histopathologically confirmed newly diagnosed breast cancer female subjects as well as 75 healthy controls were included. Blood samples of subjects were collected in serum vial and total RNA from serum was isolated by using Trizol reagent. Total RNA was polyadenylated and reverse transcribed into cDNA. Expression level of miR‐200c was detected by using miRNA qRT‐PCR. Relative expression was determined with matched controls using U6 snRNA as reference. Levels of miRNA expression was compared with distinct clinicopathological features. Total follow up period was 41 months and mean follow up time was 28 months. Kaplan Meier survival testing was performed to calculate the overall survival of breast cancer patients. The study was ethically approved by Institutional Ethics Committee, Maulana Azad Medical College, New Delhi.

Results

The overall relative fold increase of miR‐200c expression was 8.80-fold in breast cancer patients compared to the healthy controls. Level of cell free miR‐200c expression was compared with distinct clinicopathological features. There was a significant association was found between miR‐200c expression with TNM stage (p = 

Conclusions

The study revealed that the cell-free miR‐200c overexpression may be a useful, noninvasive, prognostic and predictive biomarker for breast cancer patients. A large pool study would be necessary to confirm our findings.

Clinical trial identification

NA

Legal entity responsible for the study

Dr Alpana Saxena

Funding

None

Disclosure

All authors have declared no conflicts of interest.

This site uses cookies. Some of these cookies are essential, while others help us improve your experience by providing insights into how the site is being used.

For more detailed information on the cookies we use, please check our Privacy Policy.

Customise settings