Oops, you're using an old version of your browser so some of the features on this page may not be displaying properly.

MINIMAL Requirements: Google Chrome 24+Mozilla Firefox 20+Internet Explorer 11Opera 15–18Apple Safari 7SeaMonkey 2.15-2.23

Poster presentation 1

527 - HBx upregulated lncRNA facilitates HCC tumor growth via suppression of p27Kip1/CDK2


19 Dec 2015


Poster presentation 1


Jiao-jiao Hu


Annals of Oncology (2015) 26 (suppl_9): 42-70. 10.1093/annonc/mdv523


J. Hu, X.M. Qiu, F.C. Qiao, W. Song, H.Z. Wu, P.H. Gong, X.H. Shen, H. Fan

Author affiliations

  • Department Of Medical Genetics And Developmental Biology, Medical School Of Southeast University, The Key Laboratory Of Developmental Genes And Human Diseases, Ministry Of Education, Southeast University, Southeast University, Nanjing, China, 210009 - Nanjing/CN


Abstract 527


The hepatitis B virus X protein (HBx) has been implicated in HBV-associated development of hepatocellular carcinom (HCC) by both epigenetic modifications and genetic regulations. Recently, long noncoding RNAs (lncRNAs) were found to be dysregulated in a variety of tumors and play critical regulatory roles in cancer biology. In the present study, we aimed to investigate the effects of HBx on lncRNAs expression and the underlying molecular mechanisms in hepatocarcinogenesis.


From differential expression profile of lncRNAs induced by HBx, lncRNA HBAL, which is HBx associated lncRNA, was a candidate to assess its potential function by silencing and enforced expressing lncRNA in vitro and in vivo. Cell proliferation ability of transfected cells was detected by CCK-8 assays, and cell-cycle and apoptosis were measured by flow cytometry analysis. Interaction of lncRNA HBAL with EZH2 was determined by RIP assay. The binding of EZH2 and 3MeK27H3 level in the exon 1 of p27 were measured through CHIP assay. RNA fractions of nuclear and cytosolic were separated using the PARIS Kit.


Among of upregulated 379 and downregulated 724 lncRNAs induced by HBx, an upregulated lncRNA induced by HBx was selected to explore its function in HBV-related hepatocellular carcinogenesis. In HCC tissues, the expression levels of HBAL is positively associated with HBx indicates its important roles in HCC. Enforced HBAL promotes HCC cells growth through altering cell apoptosis rate by caspase 3,8 cleaved and increase G1/S transition in vitro. In animal model, knockdown of lncRNA HBAL significantly inhibited the HCC tumor growth in vivo. Screening cell cycle regulators expression profile, CDK2 was elevated in HBAL transfected cells, while, p27, a negative regulator of CDK2, was epigenetic repressed via recruiting EZH2, a key component of PRC2. An inverse correlation between the mRNA expression of p27 and lncRNA HBAL was observed in HCC tissues.


This study is the first time report that HBx upregulates lncRNA HBAL expression to promote tumor cells growth, indicating that lncRNA HBAL is a potential oncogenic lncRNA involved in tumor progression. These data provides a broader perspective into the role of HBx in hepatocarcinogenesis.


All authors have declared no conflicts of interest.

This site uses cookies. Some of these cookies are essential, while others help us improve your experience by providing insights into how the site is being used.

For more detailed information on the cookies we use, please check our Privacy Policy.

Customise settings