Association between IL-27 -964A > G gene polymorphism and IL-27p28 levels in Chinese patients with papillary thyroid cancer

Date

19 Dec 2015

Session

Poster presentation 1

Presenters

Shulong Zhang

Citation

Annals of Oncology (2015) 26 (suppl_9): 40-41. 10.1093/annonc/mdv522

Authors

S. Zhang1, Y. Wang2, J. Jia3, Q. Zhang4, Z. Ji5

Author affiliations

  • 1 Department Of General Surgery, Zhongda Hospital Southeast University, 210009 - Nanjing/CN
  • 2 Department Of Interventional Radiology & Vascular Surgery, Zhongda Hospital Southeast University, Nanjing/CN
  • 3 Department Of Surgical Oncology, The First Affiliated Hospital of Bengbu Medical College, Bengbu/CN
  • 4 Department Of Clinical Science, The First Affiliated Hospital of Bengbu Medical College, Bengbu/CN
  • 5 Department Of General Surgery, Zhongda Hospital Southeast University, Nanjing/CN
More

Resources

Aim/Background

In this study, we aim to investigate the association between a potentially functional polymorphism (rs153109, -964A > G) at the promoter of IL-27 and the risk of papillary thyroid cancer (PTC) in a Chinese population.

Methods

Genotype of IL-27 -964A > G was determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Serum IL-27p28 levels were determined using enzyme-linked immunosorbent assay (ELISA).

Results

No significant difference was noticed in the IL-27 −964A > G distribution between PTC patients and healthy controls in overall analysis. However, stratified analysis showed that patients carrying the GG genotype or G allele had significantly decreased risks for developing lymph node metastasis compared with patients carrying the AA genotype or A allele (GG vs. AA: OR = 0.42, 95% CI, 0.24-0.73; G vs. A: OR = 0.75, 95% CI, 0.59-0.94). The levels of serum IL-27p28 were significantly decreased in PTC patients compared with those in controls (P < 0.05). Furthermore, ELISA results demonstrated that the GG genotype resulted in up-regulation of IL-27p28 expression compared with those carrying AA genotype or the AG genotype among healthy controls (P < 0.05).

Conclusions

In conclusion, our results suggest that IL-27 -964A > G polymorphism may be associated with lymph node metastasis of PTC, and IL-27p28 may be used as an attractive target for PTC immunotherapy.

Clinical trial identification

Disclosure

All authors have declared no conflicts of interest.

Resources from the same session

This site uses cookies. Some of these cookies are essential, while others help us improve your experience by providing insights into how the site is being used.

For more detailed information on the cookies we use, please check our Privacy Policy.

Customise settings