Abstract 3434
Background
Liposarcoma (LPS) is one of the most common types of soft tissue sarcoma (STS), including four subtypes: well-differentiated/atypical lipomatous tumor (ALT/WDLPS), dedifferentiated liposarcoma (DDLPS), myxoid/round cell liposarcoma (MLPS/RCLPS) and pleomorphic liposarcoma (PLS). The morphological diversity of LPS reflects the heterogeneity in clinical and biological behavior and sensitivity to chemotherapy.
Methods
The study involved six patients affected by low/high grade LPS (1 ALT/WDLPS and DDLPS, 1 DDLPS, 2 MLPS, 1 RCLPS and 1 PLS). Patient-derived primary cultures were established after patient surgery. The cultures were exposed to drugs currently used (e.i. ifosfamide, epirubicin, trabectidin, eribulin) for the treatment of LPS. Drug concentrations were selected according to the human plasma peak in patients with solid tumors. Percentages of cellular survival were assessed by MMT and TUNEL assays.
Results
The results showed the sensitivity of the cultures to chemotherapy. The combination of ifosfamide and epirubicin was the most active regimen and statistically significant among cultures. Ifosfamide did not affect the survival of the cells, except in RCLPS suggesting its role in this LPS subtype. Epirubicin showed a comparable activity of the combination regimen in DDLPS (28%) and RCLPS (14%) while displayed a lesser cytotoxic effect in ALT/WDLPS and DDLPS (83%) and MLPS (65%). Trabectidin had no effect on the cellular survival in DDLPS and an equivalent activity in ALT/WDLPS and DDLPS and MPLS (61% and 65% respectively). In RCLPS exhibited an important activity (28%). Trabectidin showed a higher efficacy in ALT/WDLPS and DDLPS and a similar trend in MLPS compared to epirubicin. Eribulin displayed a comparable activity of trabectidin in ALT/WDLPS and DDLPS (70%), MLPS (68%) and RCLPS (26%) while was more active in DDLPS (83%). Finally, cellular survival of PLS was not affected by all the drugs.
Conclusions
This study provides a rationale for elucidate the role of chemotherapy for all liposarcoma subtypes using patient-derived LPS primary cultures. The improvement in the understanding of the molecular mechanisms in liposarcoma will help in selecting the appropriate treatment with a potential impact in the clinical setting.
Clinical trial identification
Legal entity responsible for the study
Toni Ibrahim.
Funding
Has not received any funding.
Editorial Acknowledgement
Disclosure
All authors have declared no conflicts of interest.
Resources from the same session
1449 - Prognostic factors associated with pathological complete response in early breast cancer patients undergoing neoadjuvant chemotherapy. The importance of Ki-67 and molecular subtype.
Presenter: Bernardo Rapoport
Session: Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care
Resources:
Abstract
4023 - The accuracy of sentinel lymph node biopsy following neoadjuvant chemotherapy in clinically node positive breast cancer patients: A single institution experience
Presenter: Narges Sistany Allahabadi
Session: Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care
Resources:
Abstract
4707 - Index BRCA1/2 testing under a multidisciplinary program
Presenter: Duarte Machado
Session: Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care
Resources:
Abstract
3393 - BRCA1/BRCA2 germline mutations and chemotherapy-related hematological toxicity in breast cancer patients
Presenter: Alex Friedlaender
Session: Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care
Resources:
Abstract
3175 - PALB2 germ-line mutations in Russian breast cancer patients: identification of recurrent alleles and analysis of phenotypic characteristics of the tumors
Presenter: Evgeny Imyanitov
Session: Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care
Resources:
Abstract
3027 - Landscape of germline mutations in hereditary breast and ovarian cancer (HBOC) patients in Russia revealed by target panel sequencing
Presenter: Elena Shagimardanova
Session: Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care
Resources:
Abstract
4963 - Lynch syndrome-associated hereditary mutations cause breast and ovarian cancer: results from Russian Heredetary Oncogenomics project
Presenter: Marat Gordiev
Session: Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care
Resources:
Abstract
2916 - Impact of deleterious germline BRCA mutations, addition of taxanes and use of adjuvant endocrine therapy (ET) on anti-müllerian hormone (AMH) levels in early breast cancer (EBC) patients treated by adjuvant chemotherapy (CT)
Presenter: Nathalie OLYMPIOS
Session: Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care
Resources:
Abstract
2400 - Germline mutation status and therapy response in patients with triple-negative breast cancer (TNBC): Results of the GeparOcto study
Presenter: Esther Pohl
Session: Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care
Resources:
Abstract
1345 - BRCA1/BRCA2 Predictive Genetic Testing in an Irish Population: A Missed Opportunity
Presenter: David O Reilly
Session: Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care
Resources:
Abstract