Abstract 2651
Background
microRNA (miRNA) constitutes a class of small non-coding RNAs, which are involved in cell proliferation, differentiation, and progression of tumors. miRNAs and their target molecules are utilized for molecular diagnosis of urothelial carcinoma. Nucleus accumbens-associated protein 1 (NACC1), one of several transcription factors, is constitutively expressed in the urothelium, wherein it regulates cell growth, senescence, autophagy, epithelial-mesenchymal transition. We previously reported that NACC1 is the target molecule of miR-331-3p and is associated with cell proliferation in prostate and cervical cancer.
Methods
Functional experiments involving miR-331-3p and its target molecule, NACC1, was analyzed using urothelial carcinoma (UC) cell lines, T24, UMUC6, and KU7. Quantitative reverse transcription polymerase chain reaction, and immunostaining were performed to evaluate the expression of miR-331-3p and NACC1 in UC derived from transurethral resection of bladder tumor (TUB-Bt) specimens.
Results
The MTS assay revealed that cell proliferation was significantly reduced after transient transfection of miR-331-3p precursor and/or NACC1 siRNA in UC cells. Cell senescence via cell cycle arrest at the G1 phase was induced by NACC1 inhibition. Immunohistochemistry with TUR-Bt specimens revealed that greater than 90% of both UC and normal urothelial cells were positive for NACC1 in contrast to no or limited expression in squamous cell carcinoma of the esophagus, cervix, and oral cavity. The NACC1 expression profile is not significantly associated with the pathological parameters including the pT stage.
Conclusions
The present results suggest that NACC1 regulated by miR-331-3p contributes to cell proliferation and is a new potential target molecule for the diagnosis and treatment of UC.
Clinical trial identification
Legal entity responsible for the study
Nara Medical University.
Funding
Has not received any funding.
Editorial Acknowledgement
Disclosure
All authors have declared no conflicts of interest.
Resources from the same session
5127 - Mismatch repair deficient rectal cancer is resistant to induction combination chemotherapy
Presenter: Andrea Cercek
Session: Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology
Resources:
Abstract
868 - Outcomes of Chemoradiotherapy plus Local Excision in Patients with Clinical T1 or T2, N0 Rectal Cancer
Presenter: Toshiyuki Suzuki
Session: Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology
Resources:
Abstract
3195 - Comparison of Immune Microenvironment between Different Neoadjuvant Radiotherapy Regimens for Rectal Cancer
Presenter: Junxin Wu
Session: Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology
Resources:
Abstract
4032 - Prognostic factors of chemo-radiotherapy efficacy in patients with locally-advanced rectal cancer
Presenter: Mikhail Fedyanin
Session: Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology
Resources:
Abstract
3163 - Association of Systemic and Local Inflammation with Prognosis in Rectal Cancer Treated with Neoadjuvant Radiotherapy
Presenter: Jinluan Li
Session: Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology
Resources:
Abstract
1135 - Early prediction of histopathological response by PET/CT after two weeks of neoadjuvant chemoradiotherapy for rectal cancer: Wishful thinking or reality?
Presenter: Yulia Kundel
Session: Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology
Resources:
Abstract
5944 - Association between obesity and efficacy of chemoradiotherapy in rectal cancer patients
Presenter: CONSUELO Diaz Romero
Session: Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology
Resources:
Abstract
4729 - Treatment Outcomes of Patients with Localized Anal Squamous Cell Carcinoma and HIV Infection: Systematic Review and Meta-Analysis
Presenter: Marcos Camandaroba
Session: Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology
Resources:
Abstract
3329 - p16 and PD-L1 expression in locoregional squamous cell carcinoma of the anal canal – A single center retrospective analysis in Japan
Presenter: Ryo Takahashi
Session: Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology
Resources:
Abstract
919 - Factors influencing conversion to resectability and survival after resection of metastases in RAS WT metastatic colorectal cancer (mCRC): a FIRE-3 analysis
Presenter: Dominik Modest
Session: Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology
Resources:
Abstract