Abstract 3289
Background
In order to metastasize, cancer cells must detach from neighboring cells and extracellular matrix. With regard to data that microRNAs (miRNAs) miR-18, miR-19, miR-21, miR-23, miR-29, miR-155, miR-181, miR-206, miR-210, miR-221/222 and miR-375 are usually overexpressed in breast cancer cells, this research aims to identify in what way the abnormality in miRNA signature can contribute to the disintegration of cell-cell contacts.
Methods
MiRNA targets within gene transcripts were predicted in silico using the TargetScan software.
Results
Targets of overexpressed miRNAs miR-18, miR-23, miR-25, miR-150, miR-181, miR-221/222 and miR-372/373 were found in transcript of CDH1 gene encoding E-cadherin. Transcripts of genes F11R and JAM3 encoding junctional adhesion molecules JAM-A and JAM-C carry targets of miRNAs miR-23, miR-29, miR-155, miR-181 and miR-221/222. Binding sites for miRNAs miR-23 and miR-150 were revealed in transcripts of TJP1 and TJP2 genes encoding tight junction proteins ZO-1 and ZO-2. MiRNAs miR-21, miR-29, miR-155 and miR-375 can silence CLDN1 gene encoding claudin 1. Up-regulated miRNAs miR-19, miR-21 and miR-155 can target transcript of CGN gene encoding cingulin. MiRNAs miR-18, miR-29, miR-155, miR-181 and miR-375 suppress OCLN gene coding occludin. Overexpressed miRNAs miR-21, miR-23, miR-29, miR-155, and miR-221/222 can target transcripts of PVRL1 and PVRL3 genes encoding nectin 1 and 3. In addition, up-regulated miRNAs can silence other genes responsible for cell-cell adhesion - CADM1/3 (encoding nectin-like molecules 2/1), CTNNA1 (alpha-catenin), CTNND1 (p120-catenin) as well as genes encoding tropomyosin 1, vinculin, alpha-actinins.
Conclusions
MiRNAs, hyperexpressed in breast cancer cells, can silence genes encoding E-cadherin and numerous other epithelial junction components. This causes disruption of cell-cell adhesion, and, in addition, affects cell polarity and contact inhibition, predetermines epithelial-mesenchymal transition, detachment, movement and invasiveness of the breast cancer cells.
Clinical trial identification
Legal entity responsible for the study
Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.
Funding
Has not received any funding.
Editorial Acknowledgement
Disclosure
The author has declared no conflicts of interest.
Resources from the same session
1159 - Molecular characterization and search for founding effects in Canarian families with Hereditary Breast and Ovarian Cancer Syndrome
Presenter: Elisenda Llabres Valenti
Session: Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology
Resources:
Abstract
3725 - Effect of the polymorphism rs2066844 of the NOD2 gene on colon cancer incidence in a high cardiovascular risk population. Modulation by gender
Presenter: Judith Begona Ramirez Sabio
Session: Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology
Resources:
Abstract
3708 - Effect of the polymorphisms rs1476413, rs1801131, rs4846052 and rs6541003 of the MTHFR gene on prostate cancer in a high cardiovascular risk population
Presenter: Judith Begona Ramirez Sabio
Session: Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology
Resources:
Abstract
5057 - Association of a genetic variant in cyclin-dependent kinase Inhibitor 2A gene with the increased risk of breast cancer
Presenter: Soodabeh Shahid Sales
Session: Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology
Resources:
Abstract
5970 - Investigating the role of HAT protein TIP60 in regulating functional dynamics of nuclear receptor PXR
Presenter: KARISHMA BAKSHI
Session: Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology
Resources:
Abstract
1360 - The role of downregulated SIRT3 expression in patients with hepatocellular carcinoma
Presenter: Hanhee Jo
Session: Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology
Resources:
Abstract
1634 - Prediction and Intervention of Colorectal Cancer Risk with Artificial Intelligent System
Presenter: Yang Ge
Session: Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology
Resources:
Abstract
4921 - Measuring the Efficiency of Cancer Care in Europe
Presenter: Rikard Althin
Session: Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology
Resources:
Abstract
1235 - Circulating cell-free DNA isolated from plasma of mesenteric veins predicts prognosis in stage II colorectal cancer patients
Presenter: Chih-Yung Yang
Session: Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology
Resources:
Abstract
5169 - _lonality of uterine carcinosarcoma as a factor of clinical prognosis.
Presenter: Natalia Levitskaya
Session: Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology
Resources:
Abstract