Abstract 3859
Background
Glioblastoma multiforme (GBM) is the grade IV glioma and the most common adult primary brain tumor. It is notorious for its heterogeneity, invasiveness and resistance to chemo- and radiotherapies. These characteristics make it a highly recurrent malignant disease with poor prognosis. The potential new target for intervention in GBM is its cancer stem cells (GSCs).
Methods
To identify an improved treatment by targeting GSCs, we screened 400 botanical extracts for their effects on cytotoxicity and colony formation ability in GBM cell lines and tumorspheres.
Results
We identified the extracts of Cerbera manghas L. as being effective inhibitors of the growth and migration of GBM cell lines and its tumorspheres. We further demonstrated that neriifolin was one of the active principles. Neriifolin effectively inhibits the growth of CD133+-GBM tumorspheres and their colony formation. The extracts of Cerbera and neriifolin both reduced AKT activation and caused cell cycle G1 arrest while reducing the protein level of the stem cell marker Sox2. Furthermore, higher doses of these treatments induced apoptosis of tumorspheres. In the mouse xenotransplantation model, neriifolin inhibited the growth of CD133+-GBM tumorsphere-derived tumors in vivo.
Conclusions
In summary, neriifolin obtained from extracts of C. manghas may serve as a drug lead compound for GBM therapies.
Clinical trial identification
Legal entity responsible for the study
Department of life Science, National Taiwan Normal University.
Funding
Ministry of Science and Technology, Taiwan, ROC.
Editorial Acknowledgement
Louise T. Chow.
Disclosure
All authors have declared no conflicts of interest.
Resources from the same session
5421 - Comparison of palonosetron and granisetron in triplet antiemetic therapy in nonmetastatic breast cancer patients receiving high emetogenic chemotherapy. A multicenter, prospective, and observational study.
Presenter: Murat Araz
Session: Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care
Resources:
Abstract
666 - Painkiller-related dizziness in malignant tumors - A systematic review
Presenter: Rainer Spiegel
Session: Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care
Resources:
Abstract
3433 - Prevalence, risk factors and management of opioid-induced constipation in cancer pain: a nationwide, cross-sectional study in Korea
Presenter: Seung Yeon Lee
Session: Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care
Resources:
Abstract
4099 - Bald is beautiful: no more. The stigma of alopecia during chemotherapy: Brindisi Oncology Dpt experience.
Presenter: Dario Loparco
Session: Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care
Resources:
Abstract
1651 - A large multi-center, randomized, double-blind, crossover study in healthy volunteers to compare pharmacokinetics and pharmacodynamics of a proposed biosimilar pegfilgrastim with EU and US reference pegfilgrastim: methodological approach
Presenter: Roumen Nakov
Session: Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care
Resources:
Abstract
6021 - Quality of Life in Breast Cancer Tunisian Women : A monocentric survey
Presenter: Soumaya Labidi
Session: Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care
Resources:
Abstract
6114 - Anticancer therapy within the last 30 days of life in a regional cancer centre
Presenter: Mike Nguyen
Session: Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care
Resources:
Abstract
5419 - Febrile Neutropenia Management And Concordance Of Institutional Protocol With Clinical Practice
Presenter: André Oliveira
Session: Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care
Resources:
Abstract
2055 - Salient features of an indigenous integrated inpatient model of delivery of supportive medicine services - a narrative review
Presenter: Rahul Arora
Session: Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care
Resources:
Abstract
1856 - Novel online drug-drug interaction resource reveals clinically relevant interactions in >20% of the searches
Presenter: Nienke Lankheet
Session: Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care
Resources:
Abstract