To explore the different expression of proteins and the acetylations between esophageal squamous carcinoma cells (ESCCs) and cancer stem-like cells (CSCs) by proteomic analysis.
The Eca109 cells were divided into CSCs group and ESCCs group by using serum-free culture and serum culture. We measured the CD44 expression levels, CCK8 cell proliferations and plate cloning formation to identify the characteristics of cancer stem cells. Furthermore, Tandem Mass Tags (TMT)-based quantitative proteomics and bioinformatic analysis were used to detect proteomics and bioinformatic analysis.
The positive rate of CD44 and CCK8 cell proliferation experience in the CSCs group were higher than ESCCs group (P < 0.05). The plate cloning formation showed that the values of D0, Dq, N and SF2 were significantly higher in the CSCs group, and the radiation sensitization ratio was 1.556. Furthermore, 5,262 proteins were identified in the two groups in total. The up-regulation of 187 proteins and down-regulation of 83 proteins were detected in CSCs group (>1.5 times). Bioinformatic analysis further revealed that those quantifiable proteins were mainly involved in multiple biological functions and metabolic processes, including steroid biosynthesis, protein processing in endoplasmic reticulum, metabolic pathways and oxidative phosphorylation pathways. In addition, 53 acetylated sites were increased and 67 acetylated sites were decreased in CSCs group (>1.5 times). Those acetylated sites were involved in the regulation of DNA metabolic process, the function of cell adhesion, glycolysis and gluconeogenic pathway.
We provides a global survey of proteins and acetylations in ESCCs and CSCs. These proteins and acetylations may be related to the radiosensitivity, recurrence and metastatic of esophageal squamous carcinoma and could be a potential new target for esophageal squamous carcinoma.
Clinical trial identification
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All authors have declared no conflicts of interest.