Abstract 3948
Background
Chemoradiotherapy represents the main treatment for non-small cell lung cancer (NSCLC), especially for the advanced lung cancer. However, the curative effect varies significantly. Many microRNAs are verified to be associated with it and microRNA signature may be a good biomarker to predict the radiosensitivity.
Methods
Genome-wide microRNA profiling was analyzed by microarray and validated by qRT-PCR in radio-resistant cell lines and their parent cell lines (A549 and PC9, the corresponding cell lines named A549-R and PC9-R). Then we used colony formation by transfecting miRNA-mimics into A549 and PC9 for functional verification. Finally, a potential microRNA signature was established by an independent set of non-small cell lung cancer (NSCLC) serum samples and validated by available corresponding formalin-fixed paraffin-embedded tissue (FFPE) samples.
Results
73 up-regulated and 24 down-regulated miRNAs were found by microarray and 11 up-regulated, 3 down-regulated and 3 non-different miRNAs were rechecked by qRT-PCR. A miRNA signature, including miR-1290, miR-2861, miR-25-5p and miR-92a-1-5p was selected for further exploration. Overexpression of miR-1290 and miR-2861 increased the radio resistance of A549 and PC9 while overexpression of miR-25-5p and miR-92a-1-5p reversed the radio resistance of A549-R and PC9-R. The four-miRNAs signature could predict the chemotherapeutic response with high accuracy, 83.4% and 79.5% in both the test (serum samples) and validation (FFPE samples) cohorts respectively.
Conclusions
It is the first report of a miRNA signature for cell lines, serum and tissues. Serum and tissue miRNAs represent novel biomarkers to predict radiotherapy response clinically and may represent potential molecular targets to sensitize resistant cancers.
Clinical trial identification
Legal entity responsible for the study
Shandong Cancer Hospital affiliated to Shandong University.
Funding
Has not received any funding.
Editorial Acknowledgement
Disclosure
All authors have declared no conflicts of interest.
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