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Poster display session: Biomarkers, Gynaecological cancers, Haematological malignancies, Immunotherapy of cancer, New diagnostic tools, NSCLC - early stage, locally advanced & metastatic, SCLC, Thoracic malignancies, Translational research

1467 - Intraoperative Fluorescent Image-Guided Detection of Esophageal Cancer in Rabbit and Patient Specimens

Date

20 Oct 2018

Session

Poster display session: Biomarkers, Gynaecological cancers, Haematological malignancies, Immunotherapy of cancer, New diagnostic tools, NSCLC - early stage, locally advanced & metastatic, SCLC, Thoracic malignancies, Translational research

Presenters

Jiyun Rho

Citation

Annals of Oncology (2018) 29 (suppl_8): viii649-viii669. 10.1093/annonc/mdy303

Authors

J. Rho1, Y.H. Quan2, B.H. Choi2, B. Kim3, K.N. Han4, Y.H. Choi4, H.K. Kim4

Author affiliations

  • 1 Biomedical Science, Korea University College of Medicine, 136-701 - Seoul/KR
  • 2 Department Of Biomedical Sciences, Korea University College of Medicine, 136-701 - Seoul/KR
  • 3 Department Of Bio-convergence, Korea University, 08308 - Seoul/KR
  • 4 Department Of Thoracic And Cardiovascular Surgery, Korea University Guro Hospital, College of Medicine,, 08308 - Seoul/KR
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Resources

Abstract 1467

Background

This is the first study, to our knowledge, aimed at assessing the feasibility of intraoperative detection of esophageal cancer (EC) after intravenous indocyanine green (ICG) injections in rabbit and patient specimens using near-infrared (NIR) fluorescence imaging.

Methods

VX2 tumors were surgically implanted in the esophageal muscular layer of 45 rabbits 2 weeks before esophagectomy. They received 1, 2, or 5 mg/kg of intravenous ICG injections 3, 6, 12, 24, or 48 hours before surgery. Twenty-five consecutive patients (21 men; age, 63.9±8.59 years) who were scheduled to undergo esophagectomy for squamous cell EC were enrolled. Five patients received neoadjuvant concurrent chemoradiotherapy (CCRT) before surgery. All patients received 1 or 2 mg/kg of ICG intravenously 6, 12, or 24 hours before surgery. The fluorescence intensity was measured in all resected rabbit and patient specimens using an NIR fluorescence imaging system on a back table after surgery.

Results

EC was successfully detected in all rabbits; the mean tumor size was 0.86±0.21 (range, 0.5–1.3) cm. Fluorescence signals were detected in all animals. The tumor-to-normal fluorescence signal ratio (TNR) in rabbits was higher between 6 (6.89±0.35) and 12 (7.53±0.26) hours at 1 mg/kg (p < 0.01), between 12 (10.59±0.41) and 24 (12.06±0.57) hours at 2 mg/kg (p < 0.01), and between 24 (14.88±0.63) and 48 (13.73±0.19) hours at 5 mg/kg (p < 0.01) of ICG than at other times. Fluorescence signals were detected in all except 4 patients without residual tumors after preoperative CCRT. One false-positive case involved no residual tumor with inflammation (TNR, 13.64). The mean tumor size in 20 patients was 3.9±2.1 (1.1–10) cm. The TNR in the patients was higher between 6 (15.22±0.59) and 12 (17.01±0.18) hours at 1 mg/kg (p < 0.01) and between 12 (18.92±0.01) and 24 (19.81±0.73) hours at 2 mg/kg (p < 0.01) of ICG than at other times.

Conclusions

NIR fluorescence imaging revealed EC 6 to 24 hours after systemic ICG injections per their doses. However, passive ICG accumulation could not help discriminate tumors with inflammation; thus, tumor-targeted fluorescence should be developed to solve this problem in the future.

Clinical trial identification

This study was approved by the Institutional Review Board, Korea University Guro Hospital (2017GR0075).

Legal entity responsible for the study

Korea University.

Funding

This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI17C0654).

Editorial Acknowledgement

This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute(KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number : HI17C0654).

Disclosure

All authors have declared no conflicts of interest.

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