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Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care

5413 - Evaluation of the MammaTyper® as a molecular predictor for pathological complete response (pCR) after neoadjuvant chemotherapy (NACT) and outcome in patients with different breast cancer (BC) subtypes

Date

22 Oct 2018

Session

Poster display session: Breast cancer - early stage, locally advanced & metastatic, CNS tumours, Developmental therapeutics, Genitourinary tumours - prostate & non-prostate, Palliative care, Psycho-oncology, Public health policy, Sarcoma, Supportive care

Presenters

Peter A. Fasching

Citation

Annals of Oncology (2018) 29 (suppl_8): viii58-viii86. 10.1093/annonc/mdy270

Authors

P.A. Fasching1, M. Laible2, K.E. Weber3, R.M. Wirtz4, C. Denkert5, K. Schlombs6, S. Schmatloch7, O. Camara8, H.J. Lück9, J. Huober10, T. Karn11, M.T. van Mackelenbergh12, F. Marme13, V. Müller14, C. Schem15, E. Stickeler16, U. Sahin17, S. Loibl18, M. Untch19

Author affiliations

  • 1 Department Of Gynecology And Obstetrics, Universitätsklinik Erlangen, 91054 - Erlangen/DE
  • 2 Gynäkologie, BioNTech Diagnostics GmbH, Mainz/DE
  • 3 Statistic, German Breast Group (GBG) Forschungs GmbH, Neu-Isenburg/DE
  • 4 Stratifyer Molecular Pathology Gmbh, STRATIFYER Molecular Pathology GmbH, 50935 - Cologne/DE
  • 5 Institute Of Pathology, Charite Berlin Mitte, 10117 - Berlin/DE
  • 6 Biontech Diagnostics Gmbh, BioNTech Diagnostics GmbH, Mainz/DE
  • 7 Brustzentrum, Elisabeth Krankenhaus, Kassel/DE
  • 8 Brustzentrum, Hufeland Klinikum, Bad Langensalza/DE
  • 9 Gynäkologie, Gynäkologisch-Onkologische Praxis, Hannover/DE
  • 10 Gynecol. & Obstetrics, Universitaetsfrauenklinik Ulm, 89075 - Ulm/DE
  • 11 Klinik Für Frauenheilkunde Und Geburtshilfe, Universitätsklinikum Frankfurt(Johannes-Wolfgang Goethe Institute), 60590 - Frankfurt am Main/DE
  • 12 Clinic For Gynecology And Obstetrics, University Hospital Schleswig-Holstein, Kiel/DE
  • 13 Gynecologic Oncology, Nationales Zentrum für Tumorerkrankungen (NCT), 69120 - Heidelberg/DE
  • 14 Department Of Gynecology, Hamburg-Eppendorf University Medical Center, Hamburg/DE
  • 15 Mammazentrum Hamburg, Hospital Jerusalem, Hamburg/DE
  • 16 Gynäkologie Und Geburtsmedizin, Uniklinik RWTH, Aachen/DE
  • 17 Management, BioNTech AG, 55131 - Mainz/DE
  • 18 Department Of Medicine And Research, German Breast Group (GBG) Forschungs GmbH, 63263 - Neu-Isenburg/DE
  • 19 Geburtshilfe Und Gynäkologie, Helios Klinikum Berlin Buch, 13125 - Berlin/DE
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Resources

Abstract 5413

Background

According to molecular and genetic features BC can be divided into subtypes which show differences in the response to systemic therapy and in long-term outcome. Thus, effective and reliable molecular analysis of tumor material at the time of BC diagnosis is needed.

Methods

Total RNA was extracted from formalin-fixed paraffin-embedded (FFPE) tumor samples of BC patients (pts) enrolled in the single arm phase II TECHNO (Untch et al. JCO 2011) and the randomised phase III PREPARE (Untch et al. Ann Oncol. 2011) trials. MammaTyper®, a molecular in vitro diagnostic RT-qPCR test, was used to assess the expression of ERBB2 (HER2), ESR1 (ER), PGR (PR) and MKI67 (Ki67) genes as continuous and binary variables using predefined cutoffs. Pts were classified into 6 intrinsic BC subtypes according to St Gallen guidelines (Goldhirsch et al. Ann Oncol. 2013). In both trials the ER, PR, HER2 and Ki67 expression was assessed by immunohistochemistry (IHC). Predefined cutoffs for increased ESR1 (+1.8 Cq) and PGR (+3.7 Cq) expression defined a subgroup of ultra-high tumors. The study aimed to evaluate the MammaTyper® test for predicting pCR (ypT0 ypN0) after NACT and outcome in the BC subtypes. The degree of agreement between the MammaTyper® and the IHC test for determining BC subtypes was also estimated.

Results

A total of 418 pts were assessed. The BC subtypes defined by MammaTyper® and IHC showed good agreements (ERBB2/HER2, kappa [κ]=0.674; ESR1/ER, κ = 0.815; PGR/PR, κ = 0.648). MKI67 significantly predicted pCR (AUC=0.686, p < 0.001). In HER2+ pts from the TECHNO trial the ERBB2 significantly predicted pCR in ESR1-positive sybtype (n = 59, AUC=0.708, p = 0.006). HER2-negative pts from the PREPARE trial with ultra-high ESR1 and/or PGR expression had significantly better disease-free (DFS) and overall survival (OS) than the non-ultra-high pts (n = 153; DFS HR = 1.80, [95%CI 1.18-2.76], p = 0.007; OS HR = 2.54 [95%-CI 1.50-4.31], p = 0.001).

Conclusions

The MammaTyper® significantly predicts response after NACT in BC subtypes. A subgroup of pts with ultra-high ESR1/PGR expression had a good prognosis. Further analysis is required.

Clinical trial identification

Legal entity responsible for the study

GBG Forschungs GmbH and BioNTech Diagnostics GmbH.

Funding

This study was financially supported by BioNTech Diagnostics GmbH, Mainz, Germany.

Editorial Acknowledgement

Disclosure

P.A. Fasching: Grants: Biontech, during the conduct of the study; Grants and personal fees: Novartis; Personal fees: Pfizer, Roche, Teva, Celgene, outside the submitted work. M. Laible: Patent WO 2015/024942, Commercialized as MammaTyper(TM) Kit; Relevant financial activities outside the submitted work: BioNTech Diagnostics GmbH (Employee of BioNTech Diagnostics GmbH). K.E. Weber: Grants and non-financial support: BioNTech Diagnostics GmbH, Mainz, Germany, during the conduct of the study; Patent EndoPredict issued. C. Denkert: Personal fees: Teva, Novartis, Pfizer, Roche, Amgen, MSD Oncology; Other: Sividon Diagnostics, outside the submitted work. K. Schlombs: Personal fees: BioNTech Diagnostics GmbH, outside the submitted work; Patent WO 2015/024942 pending; New Patent application pending. F. Marme: Personal fees: Roche, AstraZeneca, Pfizer, Tesaro, Novartis, Amgen, PharmaMar, GenomicHealth, CureVac, Eisai, outside the submitted work. S. Loibl: Grants: AbbVie, Amgen, AstraZeneca, Celgene, Novartis, Pfizer, Roche, Teva, Vifor during the conduct of the study and outside the submitted work. All other authors have declared no conflicts of interest.

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