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Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology

5720 - Evaluation of stemness and proliferation of human breast cancer stem cells (ALDH+) supplemented with heat-activated TGF-beta1 in the secretomes of stem cells from human exfoliated deciduous teeth (SHED)

Date

21 Oct 2018

Session

Poster display session: Basic science, Endocrine tumours, Gastrointestinal tumours - colorectal & non-colorectal, Head and neck cancer (excluding thyroid), Melanoma and other skin tumours, Neuroendocrine tumours, Thyroid cancer, Tumour biology & pathology

Presenters

Septelia Wanandi

Citation

Annals of Oncology (2018) 29 (suppl_8): viii1-viii13. 10.1093/annonc/mdy268

Authors

S.I. Wanandi1, G. Wideani2, M. Zainuri3, R.R. Putri4, E.W. Bachtiar5, S.W.A. Jusman6

Author affiliations

  • 1 Biochemistry And Molecular Biology, Faculty of Medicine, Universitas Indonesia, 10430 - Jakarta/ID
  • 2 Master Program In Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta/ID
  • 3 Doctoral Program In Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta/ID
  • 4 Center Of Biomedical And Basic Technology Of Health, National Institute of Health Research and Development, Ministry of Health, Indonesia, Jakarta/ID
  • 5 Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta/ID
  • 6 Biochemistry And Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta/ID
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Resources

Abstract 5720

Background

Our previous studies have reported that the secretomes of umbilical cord- and adipose-derived mesenchymal stem cells (MSCs) affected the stemness properties of human breast CSCs (BCSCs). However, little is known about specific factors in MSC secretomes, particularly those from human exfoliated deciduous teeth, which involved in tumor aggressiveness such as stemness and proliferation of CSCs. This study aimed to evaluate the stemness and proliferation of human BCSCs after supplemented with heated secretomes of stem cells from human exfoliated deciduous teeth (SHED) to activate latent TGF-β1.

Methods

To collect SHED conditioned medium (SHED-CM) containing secretomes, SHED were grown in serum-free a-MEM for 24 and 48 hours, respectively. SHED-CM 24-h was then heated at 800C for 10 min. Human BCSCs (ALDH+) cultured in DMEM-F12 were supplemented with 50% (v/v) non-heated SHED-CM 24- and 48-h, as well as with heated SHED-CM 24-h followed by 72-h incubation. Control was BCSCs supplemented with non-heated 50% (v/v) a-MEM/DMEM-F12. Following the supplementation, we measured the mRNA expression of TGF-β1 receptor (TβRI), as well as stemness genes ALDH1A1 and OCT4 of BCSCs using qRT-PCR. BCSC proliferation was determined using trypan blue dye.

Results

This study demonstrates that relative mRNA expression levels of TβRI, OCT4 and ALDH1A1 in BCSCs supplemented with non-heated SHED-CM 24- and 48-h were increased compared to their control. Interestingly, the increase of TβRI, OCT4 and ALDH1A1 expressions after TGF-β1 heat activation was significantly higher than in non-heated SHED-CM. Conversely, BCSC proliferation was significantly reduced after supplemented with non-heated SHED-CM 24- and 48-h, but drastically increased higher than control when treated with heated SHED-CM 24-h, suggesting the involvement of other factors in SHED-CM that restrain TGF-β1 signaling and suppress cell proliferation.

Conclusions

Heated SHED secretomes contained activated TGF-β1 that increased the expression of stemness genes, OCT4 and ALDH1A1, as well as proliferation of human BCSCs (ALDH+) via TGF-β1 paracrine signaling.

Clinical trial identification

Legal entity responsible for the study

Septelia Inawati Wanandi.

Funding

Directorate of Research and Community Service, Universitas Indonesia.

Editorial Acknowledgement

Disclosure

All authors have declared no conflicts of interest.

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