Oops, you're using an old version of your browser so some of the features on this page may not be displaying properly.

MINIMAL Requirements: Google Chrome 24+Mozilla Firefox 20+Internet Explorer 11Opera 15–18Apple Safari 7SeaMonkey 2.15-2.23

Poster display session

1676 - Intratumoral heterogeneity of SMAD4 immunohistochemical (IHC) expression and its role in prediction of recurrence patterns in patients with resectable pancreatic cancer (PC)


09 Sep 2017


Poster display session


Translational Research;  Pancreatic Cancer


Ilya Pokataev


Annals of Oncology (2017) 28 (suppl_5): v209-v268. 10.1093/annonc/mdx369


I. Pokataev1, A. Kudaibergenova2, A. Artemyeva3, D. Podluzhnyi4, N. Kudashkin4, Y. Patyutko4, E. Moroz5, E. Kharchenko3, A. Popova1, M. Fedyanin1, A. Rumyantsev1, I. Bazin1, A. Tryakin1, E. Glukhov6, O. Sekhina1, D. Chekini1, S. Tjulandin1

Author affiliations

  • 1 Department Of Clinical Pharmacology And Chemotherapy, N. N. Blokhin Russian Cancer Research Center, 115478 - Moscow/RU
  • 2 Department Of Cytology, N. N. Blokhin Russian Cancer Research Center, 115478 - Moscow/RU
  • 3 Department Of Morphology, Petrov Institute of Oncology, Saint Petersburg/RU
  • 4 Department Of Surgery #7, N. N. Blokhin Russian Cancer Research Center, 115478 - Moscow/RU
  • 5 Department Of Morphology, N. N. Blokhin Russian Cancer Research Center, 115478 - Moscow/RU
  • 6 Department Of Surgery, N. N. Blokhin Russian Cancer Research Center, 115478 - Moscow/RU


Abstract 1676


Up to 30% of patients with resectable PC have only locoregional recurrences and never experience metastatic disease. Several authors reported SMAD4 expression can predict locoregional pattern of PC progression. The aim of our study was to evaluate consistency of SMAD4 expression in different tumor areas and its correlation with patterns of PC recurrence.


Records of PC patients treated in N.N. Blokhin Russian Cancer Research Center since 2002 to 2015 were analyzed. Inclusion criteria for this retrospective analysis were: nonmetastatic morphologically confirmed PC, R0-R1 resection and archive tumor samples availability. Formalin-fixed, paraffin-embedded tissue sections of different areas of the primary tumor (central area and zones of invasion) and of lymph node metastases were analyzed via IHC for SMAD4 expression using TMA technology.


A total of 356 tissue sections obtained from 91 patients were assessed for SMAD4 expression. Positive SMAD4 expression was revealed in tumor blocks of 26 patients. There was high intratumoral heterogeneity of SMAD4 expression: only in 4 of 26 patients (15.4%) SMAD4 expression was positive in all assessed tumor slides. There were 54 recurrences (9 locoregional, 41 distant and 4 both local and distant) with median follow-up 21.7 months. There were no correlation between SMAD4 expression and locoregional recurrence pattern (Goodman & Kruskal's tau coefficient 0.08±0.03, p = 0,13). There was no difference in distant recurrence-free survival by SMAD4 IHC expression status: medians were 11.8, 19.5 and 7.1 months for patients with SMAD4 negative, heterogeneous or positive tumors, respectively (p = 0.987). SAMD4 status also showed no prognostic significance: medians overall survival were 20.5, 32.6 and 15.2 months for patients with SMAD4-positive, heterogeneous and negative tumors, respectively (p = 0.131).


Different areas inside the primary tumor and lymph node metastases heterogeneously express SMAD4. SMAD4 IHC expression is not a biomarker of a recurrence pattern following surgical resection for PC.

Clinical trial identification

Legal entity responsible for the study

N.N. Blokhin Russian Cancer Research Center


N.N. Blokhin Russian Cancer Research Center


All authors have declared no conflicts of interest.

This site uses cookies. Some of these cookies are essential, while others help us improve your experience by providing insights into how the site is being used.

For more detailed information on the cookies we use, please check our Privacy Policy.

Customise settings
  • Necessary cookies enable core functionality. The website cannot function properly without these cookies, and you can only disable them by changing your browser preferences.