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Poster display session

5177 - Epidermal growth factor receptor (EGFR) copy number (CN) as a biomarker of prognosis and panitumumab (Pan) benefit in RAS-wt advanced colorectal cancer (aCRC)


09 Sep 2017


Poster display session


Cytotoxic Therapy;  Translational Research;  Colon and Rectal Cancer


Jenny Seligmann


Annals of Oncology (2017) 28 (suppl_5): v158-v208. 10.1093/annonc/mdx393


J. Seligmann, H. Wood, S. Richman, F. Elliott, M. Taylor, E. Tinkler-Hundal, J. Barrett, M. Seymour, P. Quirke

Author affiliations

  • Leeds Institute Of Cancer And Pathology, University of Leeds, LS9 - Leeds/GB


Abstract 5177


Whilst RAS mutations predict which aCRC patients (pts) will not benefit from anti-EGFR agents, RAS-wt status does not reliably predict who will. Several studies report that high EGFR ligand expression (EREG/AREG) is predictive of anti-EGFR agent benefit but progression towards clinical utility is limited by lack of consensus on a clinical dichotomisation point and additional tumor material required. Here we explore EGFR copy number as a biomarker of prognosis and predictor of Pan benefit in a randomised trial in aCRC.


EGFR CN, EREG/AREG RNA expression, RAS/RAF mutations were assessed in tumor from 275 pts randomised to 2nd-line irinotecan (Ir) or IrPan (PICCOLO, Lancet Onc 14:749-59). EGFR CN status was measured by the Affymetrix OncoScan array, analysed using Biodiscovery Nexus software and defined as normal (2 copies) or gain (>2 copies). Prognostic analysis was in Ir alone pts. Predictive analysis, in the 234 RAS-wt pts, compared baseline values with outcomes using Cox proportional hazards models.


196 (71.3%) pts were classified as EGFR gain and 79 (28.7%) as normal. EGFR gain was significantly associated with high EREG and AREG RNA expression (both p 


EGFR CN status may allow for further stratification for Pan benefit in RAS-wt patients. Normal EGFR CN status identified nearly 1/3 of pts without Pan benefit. This biomarker is consistent with EGFR ligand data, and is a DNA-based assay with a clearly definable dichotomised cut-point and therefore is of potential clinical utility.

Clinical trial identification


Legal entity responsible for the study

University of Leeds




All authors have declared no conflicts of interest.

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