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Poster display session

3956 - Eph A2 expression is a predictive biomarker of poorer activity and efficacy of FOLFIRI + cetuximab in RAS WT metastatic colorectal cancer (mCRC) patients (pts) in the CAPRI GOIM trial


11 Sep 2017


Poster display session


Cytotoxic Therapy;  Translational Research;  Colon and Rectal Cancer


Claudia Cardone


Annals of Oncology (2017) 28 (suppl_5): v573-v594. 10.1093/annonc/mdx390


C. Cardone1, M.C. Paul2, V. Moreno-Viedma2, G. Martini1, P.P. Vitiello1, D. Ciardiello1, V. Sforza1, T. Troiani1, S. Napolitano1, P. Vitale1, N. Zanaletti1, A.M. Rachiglio3, D. Rizzi4, E. Maiello5, N. Normanno6, M. Sibilia2, F. Ciardiello1, E. Martinelli1

Author affiliations

  • 1 Oncologia Medica, Dipartimento Di Internistica Clinica E Sperimentale “f. Magrassi”,, Università degli Studi della Campania Luigi Vanvitelli, 80121 - Napoli/IT
  • 2 Department Of Medicine I, Institute of Cancer Research, Vienna/AT
  • 3 Laboratory Of Pharmacogenomics, Centro Di Ricerche Oncologiche Di Mercogliano (crom), National Cancer Institute ‘Fondazione Giovanni Pascale', 80121 - Napoli/IT
  • 4 Trial Office, GOIM, Bari/IT
  • 5 Oncologia, IRCCS Casa Sollievo della Sofferenza, 71013 - San Giovanni Rotondo/IT
  • 6 Cell Biology Unit, Istituto Nazionale Tumori – I.R.C.C.S - Fondazione Pascale, 80131 - Napoli/IT


Abstract 3956


Eph A2 promotes tumor growth, invasiveness and angiogenesis in mCRC. Targeting Eph A2 could overcome resistance to anti-epidermal growth factor receptor treatment in colon cancer preclinical models.


Formalin-fixed paraffin-embedded tumor specimens from 82 RAS wild type (WT) mCRC pts treated with cetuximab + FOLFIRI as first line therapy in the CAPRI GOIM trial were assessed for Eph A2 expression by immunohistochemistry. Eph A2 levels were evaluated developing an HSCORE [1 × (% cells 1+) + 2 × (% cells 2+) + 3 × (% cells 3+)] (range: 0-300). A cut off was set by ROC analysis to define high (>50) and low (≤50) Eph A2 levels.


Eph A2 expression was found in 55/82 (67%) cases. According to HSCORE Eph A2 levels were low in 54 (66%) and high in 28 (34%) samples. Eph A2 expression resulted in mostly complete membranous staining. Tumor stroma was positive in 15/82 (18%) cases. In most of these cases an intense immune infiltrate was observed. Non-tumor adjacent normal mucosa was assessable in 34/82 samples. Eph A2 was expressed in 16/34 (47%), more frequently in dysplastic epithelial areas. A significant correlation between Eph A2 expression in tumor and stroma was found (p 


Eph A2 levels were significantly associated with a worse PFS and an increase in PD in RAS WT mCRC pts treated with cetuximab + FOLFIRI as first line therapy in the CAPRI GOIM trial, in both right- and left-sided tumors. A similar trend was observed for OS. Eph A2 might represent an additional predictive biomarker of lack of efficacy in RAS WT mCRC pts treated with cetuximab + FOLFIRI.

Clinical trial identification

Legal entity responsible for the study

Department of Clinical and Experimental Medicine ‘F. Magrassi’ Università degli studi della Campania “Luigi Vanvitelli”, Naples, Italy.




F. Ciardiello: Advisory boards: Merck Serono, Lilly, Roche, Bayer, Amgen, Pfizer. E. Martinelli: Advisory boards: Merck Serono, Amgen. All other authors have declared no conflicts of interest.

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