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Poster display session

4225 - Characterization of breast cancer responses to metformin

Date

11 Sep 2017

Session

Poster display session

Topics

Breast Cancer

Presenters

Aesha Makhtar

Citation

Annals of Oncology (2017) 28 (suppl_5): v74-v108. 10.1093/annonc/mdx365

Authors

A. Makhtar

Author affiliations

  • Oncology Department, University of Sheffield, S10 2rx - Sheffield/GB
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Resources

Abstract 4225

Background

Over the last six decades, metformin has become one of the most widely prescribed oral medications for type II diabetes. It has recently received considerable attention because of its potential role in reducing the risk of cancer development and its antineoplastic properties. However, the mechanism behind the growth-inhibitory effect of metformin on breast cancer cells remains unclear, with little consensus on which tumour subtypes benefit from treatment. Furthermore, it should be noted that much of the in vitro work published to date has used drug concentrations greatly exceeding the recommended clinical dose, and therefore may not translate directly into clinical practice.

Methods

Non-tumorigenic (MCF10A), pre-malignant (MCF10AT), pre-invasive (DCIS), the three-invasive breast cancer [MCF7, T47D and MDA-MB-231] and the fully bone-homed variant of MDA-MB-231(BM) were treated with metformin and effects on cellular proliferation were evaluated by trypan-blue exclusion and clonogenic assays. The expression levels of metformin transporters mRNA and proteins (OCT1-3, MATE1-2 and PMAT) were evaluated by qRT-PCR, western blot.

Results

The growth-curtailing effect of metformin was achieved at 0.3mM for MDA-MB-231(P = 0.0198) in cell counting assays. Colony-forming capacity was inhibited in all cell lines tested at 0.03mM, (P 

Conclusions

Clinically relevant doses of metformin inhibited the proliferation and colony formation of different breast cancer subtypes regardless of their receptor status and aggressiveness, including the hard to treat triple negative subtype MDA-MB-231 cells. Expression of various influx and efflux metformin-transporters is essential for cellular response and sensitivity to treatment.

Clinical trial identification

Legal entity responsible for the study

Libyan higher ministry of education

Funding

None

Disclosure

All authors have declared no conflicts of interest.

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